• Product NameRecombinant human SUMO-conjugating enzyme UBC9
• Brief DescriptionRecombinant Protein
• Host SpeciesE.coli
• PurificationGreater than 90% as determined by SDS-PAGE.
• Immunogen DescriptionExpression Region:1-157aa
  Sequence Info:Partial
• Alternative Names    SUMO-protein ligaseUbiquitin carrier protein 9Ubiquitin carrier protein IUbiquitin-conjugating enzyme E2 IUbiquitin-protein ligase Ip18
• Accession No. P63279
• UniprotP63279
• Gene ID7329;
• Calculated MW 44.9 kDa
• Tag Info N-terminal GST-tagged
• Target Sequence MSGIALSRLAQERKAWRKDHPFGFVAVPTKNPDGTMNLMNWECAIPGKKGTPWEGGLFKLRMLFKDDYPSSPPKCKFEPPLFHPNVYPSGTVCLSILEEDKDWRPAITIKQILLGIQELLNEPNIQDPAQAEAYTIYCQNRVEYEKRVRAQAKKFAP
• Formulation Tris-based buffer50% glycerol
• Storage The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20˚C,-80˚C. The shelf life of lyophilized form is 12 months at -20˚C,-80˚C.
  Notes:Repeated freezing and thawing is not recommended. Store working aliquots at 4˚C for up to one week.

Accepts the ubiquitin-like proteins SUMO1, SUMO2, SUMO3 and SUMO4 from the UBLE1A-UBLE1B E1 complex and catalyzes their covalent attachment to other proteins with the help of an E3 ligase such as RANBP2 or CBX4. Can catalyze the formation of poly-SUMO chains. Necessary for sumoylation of FOXL2 and KAT5. Essential for nuclear architecture and chromosome segregation. Sumoylates p53,TP53 at 'Lys-386'

Identification of the structural and functional human homolog of the yeast ubiquitin conjugating enzyme UBC9.Yasugi T., Howley P.M.Nucleic Acids Res. 24:2005-2010(1996)
Research Topic:Cell Cycle

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting