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EGFR(Phospho-Tyr869) Antibody #11229

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Catalog No. Package Size Price Quantity
50ul $219.00
100ul $319.00
Availability:Yes
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Description
  • Product NameEGFR(Phospho-Tyr869) Antibody
  • Host SpeciesRabbit
  • ClonalityPolyclonal
  • PurificationAntibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
  • ApplicationsWB
  • Species ReactivityHu Ms Rt
  • SpecificityThe antibody detects endogenous level of EGFR only when phosphorylated at tyrosine 869.
  • Immunogen TypePeptide-KLH
  • Immunogen DescriptionPeptide sequence around phosphorylation site of tyrosine 869 (K-E-Y(p)-H-A) derived from Human EGFR.
  • Target NameEGFR
  • ModificationPhospho
  • Alternative Names    ERBB1; Receptor protein-tyrosine kinase ErbB-1; kinase EGFR
  • Accession No. Swiss-Prot: P00533
    NCBI Protein: NP_005219.2
  • UniprotP00533
  • Gene ID1956;
  • Concentration 1.0mg/ml
  • Formulation Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
  • Storage Store at -20°C for long term preservation (recommended). Store at 4°C for short term use.
Application Details
Background

Published Papers

Mutual regulation between phosphofructokinase 1 platelet isoform and VEGF promotes glioblastoma tumor growth. In Cell Death Dis on 2022 Nov 26 by Je Sun Lim, YuJie Shi, et al..PMID:36435833, , (2022),
PMID: 36435833
EGFR-Phosphorylated Platelet Isoform of Phosphofructokinase 1 Promotes PI3K Activation. IN Mol Cell.On 2018 Apr 19 by Lee JH, Liu R et al..PMID:29677490, , (2018),
PMID: 29677490
Secreted and O-GlcNAcylated MIF binds to the human EGF receptor and inhibits its activation.In Nat Cell Biol on 2015 Oct by Yanhua Zheng, Xinjian Li et al..PMID:26280537 , , (2015),
PMID: 26280537
Please let us know if you have published research using #11229 so that we can cite your reference.

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Application

WBWestern Blotting

IHCImmunohistochemistry

IFImmunofluorescence

ICCImmunocytochemistry

FCFlow Cytometry

IPImmunoprecipitation

EELISA

DBDot Blotting

ChIPChromatin Immunoprecipitation

GICAGold Immunochromatography Assay

NCNegative Control

Species Reactivity

HuHuman

MsMouse 

RtRat 

Dm Drosophila melanogaster

C Caenorhabditis elegans

MkMonkey

RbRabbit

B Bovine 

D Dog

PPig

HmHamster

ChHm Chinese Hamster 

ChkChicken  

ShpSheep