• Product NameRecombinant mouse C-C motif chemokine 2
• Brief DescriptionRecombinant Protein
• Host SpeciesE.coli
• PurificationGreater than 90% as determined by SDS-PAGE.
• Immunogen DescriptionExpression Region:24-96aa
  Sequence Info:Partial
• Alternative Names    HC11Monocyte chemoattractant protein 1Monocyte chemotactic and activating factor ;MCAFMonocyte chemotactic protein 1 ;MCP-1Monocyte secretory protein JESmall-inducible cytokine A2
• Accession No. P10148
• UniprotP10148
• Gene ID20296;
• Calculated MW 12.4 kDa
• Tag Info N-terminal 6xHis-tagged
• Target Sequence QPDAINAPVTCCYNFTNRKISVQRLASYRRITSSKCPKEAVIFKTIVAKEICADPKQKWVQDSMDHLDKQTQT
• Formulation Tris-based buffer50% glycerol
• Storage The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20˚C,-80˚C. The shelf life of lyophilized form is 12 months at -20˚C,-80˚C.
  Notes:Repeated freezing and thawing is not recommended. Store working aliquots at 4˚C for up to one week.

Chotactic factor that attracts monocytes and basophils but not neutrophils or eosinophils. Augments monocyte anti-tumor activity. Has been implicated in the pathogenesis of diseases characterized by monocytic infiltrates, like psoriasis, rheumatoid arthritis or atherosclerosis. May be involved in the recruitment of monocytes into the arterial wall during the disease process of atherosclerosis.

"Deletion of the NH2-terminal residue converts monocyte chemotactic protein 1 from an activator of basophil mediator release to an eosinophil chemoattractant."Weber M., Uguccioni M., Baggiolini M., Clark-Lewis I., Dahinden C.A.J. Exp. Med. 183:681-685(1996)
Research Topic:Others

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting