• Product NameTLR3 Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationIon exchange chromatography purified
• ApplicationsELISA WB ICC
• Species ReactivityHu Ms
• Immunogen TypePeptide
• Immunogen DescriptionRaised against a peptide corresponding to 15 amino acids near the carboxy terminus of human TLR3.
• Target NameTLR3
• Alternative Names    Toll-like receptor 3, CD283
• Accession No. O15455
• UniprotO15455
• Gene ID7098;
• Concentration 1mg/ml
• Formulation Supplied in PBS containing 0.02% sodium azide.
• Storage Can be stored at -20˚C, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Toll-like receptors (TLRs) are evolutionarily conserved pattern-recognition molecules resembling the toll proteins that mediate antimicrobial responses in Drosophila. These proteins recognize different microbial products during infection and serve as an important link between the innate and adaptive immune responses. The TLRs act through adaptor molecules such as MyD88 and TIRAP to activate various kinases and transcription factors so the organism can respond to potential infection. TLR3 is known to recognize viral double-stranded (ds) RNA, a molecular pattern associated with viral infection. Recently it has been shown to recognize viruses such as Influenza A and West Nile Virus and can mediate entry of at least West Nile Virus.

• WB Validation in Human CellsLoading: 15 ug of lysate Antibodies: TLR3, 1 ug/mL , 1 h incubation at RT in 5% NFDM/TBST. Secondary: Goat Anti-Rabbit IgG HRP conjugate at 1:10000 dilution.
• WB Validation in Mouse and Rat BrainLoading: 15 ug of lysate Antibodies: TLR3, 1 ug/mL , 1 h incubation at RT in 5% NFDM/TBST. Secondary: Goat Anti-Rabbit IgG HRP conjugate at 1:10000 dilution.
• WB Validation in Human Daudi CellsLoading: 10 ug of lysate Antibodies: TLR3, 1 ug/mL (A) and 2 ug/mL (B), 1 h incubation at RT in 5% NFDM/TBST. Secondary: Goat Anti-Rabbit IgG HRP conjugate at 1:10000 dilution.
• Immunofluorescence Validation of TLR3 in Human Spleen Immunofluorescent analysis of 4% paraformaldehyde-fixed human spleen tissue labeling TLR3 at 10 ug/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (red) and DAPI staining (blue).
• Immunofluorescence Validation of TLR3 in Mouse Spleen Immunofluorescent analysis of 4% paraformaldehyde-fixed mouse spleen tissue labeling TLR3 at 20 ug/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (green) and DAPI staining (blue).
• Immunohistochemistry Validation of TLR3 in Mouse Brain Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-TLR3 antibody at 1 ug/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4°C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.
• Immunohistochemistry Validation of TLR3 in Rat Brain Immunohistochemical analysis of paraffin-embedded rat brain tissue using anti-TLR3 antibody at 1 ug/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4°C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting