• Product Namec-Kit(Phospho-Tyr721) Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationAntibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
• ApplicationsWB
• Species ReactivityHu
• SpecificityThe antibody detects endogenous level of c-Kit only when phosphorylated at tyrosine 721.
• Immunogen TypePeptide-KLH
• Immunogen DescriptionPeptide sequence around phosphorylation site of tyrosine 721 (N-E-Y(p)-M-D) derived from Human c-Kit.
• Target Namec-Kit
• ModificationPhospho
• Alternative Names    KIT , CD117, SCFR, PBT
• Accession No. Swiss-Prot: P10721
  NCBI Protein: NP_000213.1
• UniprotP10721
• Gene ID3815;
• Concentration 1.0mg/ml
• Formulation Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Storage Store at -20°C for long term preservation (recommended). Store at 4°C for short term use.

• Predicted MW: 145kd
  Western blotting: 1:500~1:1000
  

This is the receptor for stem cell factor (mast cell growth factor). It has a tyrosine-protein kinase activity. Binding of the ligands leads to the autophosphorylation of KIT and its association with substrates such as phosphatidylinositol 3-kinase (Pi3K)

Nocka, K. et al. (1990) EMBO J. 9, 1805-1813.
Hirota, S. et al. (1998) Science 279, 577-580.
Blume-Jensen, P. et al. (2000) Nat. Genet. 24, 157-162.
Sattler, M. et al. (1997) J. Biol. Chem. 272, 10248-10253.
Gommerman, J.L. et al. (1997) J. Biol. Chem. 272, 30519-30525.

• Western blot analysis of extracts from Hela cells untreated or treated with IFN-a using c-Kit(Phospho-Tyr721) Antibody #11240.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting