• Product NameRecombinant mouse Tryptase
• Brief DescriptionRecombinant Protein
• Host SpeciesYeast
• PurificationGreater than 90% as determined by SDS-PAGE.
• Immunogen DescriptionExpression Region:35-267aa
  Sequence Info:Partial
• Alternative Names    Mast cell protease 7 ;mMCP-7Tryptase alpha,beta-1
• Accession No. Q02844
• UniprotQ02844
• Gene ID100503895;
• Calculated MW 28.1 kDa
• Tag Info N-terminal 6xHis-tagged
• Target Sequence AHGNKWPWQVSLRANDTYWMHFCGGSLIHPQWVLTAAHCVGPDVADPNKVRVQLRKQYLYYHDHLMTVSQIITHPDFYIVQDGADIALLKLTNPVNISDYVHPVPLPPASETFPSGTLCWVTGWGNIDNGVNLPPPFPLKEVQVPIIENHLCDLKYHKGLITGDNVHIVRDDMLCAGNEGHDSCQGDSGGPLVCKVEDTWLQAGVVSWGEGCAQPNRPGIYTRVTYYLDWIHH
• Formulation Tris-based buffer50% glycerol
• Storage The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20˚C,-80˚C. The shelf life of lyophilized form is 12 months at -20˚C,-80˚C.
  Notes:Repeated freezing and thawing is not recommended. Store working aliquots at 4˚C for up to one week.

Tryptase is the major neutral protease present in mast cells and is secreted upon the coupled activation-degranulation response of this cell type.

Isolation, characterization, and transcription of the gene encoding mouse mast cell protease 7.McNeil H.P., Reynolds D.S., Schiller V., Ghildyal N., Gurley D.S., Austen K.F., Stevens R.L.Proc. Natl. Acad. Sci. U.S.A. 89:11174-11178(1992)
Research Topic:Others

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting