• Product NameRecombinant Human Fibroblast growth factor 2 protein(FGF2) (Active)
• Brief DescriptionRecombinant Protein
• Purification>96% as determined by SDS-PAGE and HPLC.
• Biological ActivityCell Based Assay Measured in a cell proliferation assay using BALB/c 3T3 cells.The ED50 for this effect is 0.42 ng/ml(Regularly tested).
• Alternative Names    FGF-2, bFGF, Heparin-binding growth factor 2, HBGF-2,
• Accession No. P09038
• UniprotP09038
• Gene ID2247;
• Calculated MW 17.4 KDa
• SDS-PAGE MW 16 KDa, reducing conditions
• Formulation Lyophilized from a 0.2 μm filtered solution of 20mM Tris, 150mM NaCl, 3% Trehalose, 4% Mannitol, pH 7.5
• Storage Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt.
  Reconstituted protein solution can be stored at 2-8°C for 2-7 days.
  Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months.

Plays an important role in the regulation of cell survival, cell division, angiogenesis, cell differentiation and cell migration. Functions as potent mitogen in vitro. {ECO:0000269|PubMed:1721615, ECO:0000269|PubMed:8663044}.

PubMed ID: 3472745; 3780670; 2538817; 19054851; 15815621; 1785797; 2435575; 1721615; 8564983; 3579930; 2435284; 3964259; 3732516; 1417798; 1885605; 8663044; 10358027; 11509569; 11964394; 16257968; 18669637; 20230531; 15863030; 20094046; 22321063; 1769963; 1707542; 1849658; 1702556; 7691311; 11390973; 8885834
Research Topic:Cancer

• SDS-PAGE:Greater than 95% as determined by reducing SDS-PAGE.
• Cell Based Assay:Measured in a cell proliferation assay using BALB/c 3T3 cells.The ED50 for this effect is 0.42 ng/ml(Regularly tested).

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting