• Product NameRecombinant Human Insulin-like growth factor I protein(IGF1) (Active)
• Brief DescriptionRecombinant Protein
• Purification>97% as determined by SDS-PAGE and HPLC.
• Biological ActivityFully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using serum free human MCF-7 cells is less than 2 ng,ml, corresponding to a specific activity of 5.0 105 IU,mg.
• Immunogen DescriptionExpression Region:49-118aa
  Sequence Info:Full Length of Mature Protein
• Alternative Names    IGF-I, MGF, Somatomedin-C
• Accession No. P05019
• UniprotP05019
• Gene ID3479;
• Calculated MW 7.7 kDa
• Tag Info NO-tagged
• Target Sequence GPETLCGAEL VDALQFVCGD RGFYFNKPTG YGSSSRRAPQ TGIVDECCFR SCDLRRLEMY CAPLKPAKSA
• Formulation 0.2 ?m filtered PBS, pH 7.0 ,lyophilized
• Storage The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20˚C,-80˚C. The shelf life of lyophilized form is 12 months at -20˚C,-80˚C.
  Notes:Repeated freezing and thawing is not recommended. Store working aliquots at 4˚C for up to one week.

The insulin-like growth factors, isolated from plasma, are structurally and functionally related to insulin but have a much higher growth-promoting activity. May be a physiological regulator of [1-14C]-2-deoxy-D-glucose (2DG) transport and glycogen synthesis in osteoblasts. Stimulates glucose transport in rat bone-derived osteoblastic (PyMS) cells and is effective at much lower concentrations than insulin, not only regarding glycogen and DNA synthesis but also with regard to enhancing glucose uptake. May play a role in synapse maturation. {ECO:0000269|PubMed:21076856, ECO:0000269|PubMed:24132240}.

PubMed ID: 6358902; 3002851; 2935423; 2937782; 3455760; 2082190; 2018498; 1372070; 8495408; 16541075; 15489334; 6382022; 632300; 3242681; 21076856; 24132240; 6189745; 2036417; 1319992; 8857020; 10391209
Research Topic:Cancer

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting