• Product NameP38 MAPK(Phospho-Thr180) Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationAntibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
• ApplicationsWB IHC
• Species ReactivityHu Ms Rt
• SpecificityThe antibody detects endogenous level of P38 MAPK only when phosphorylated at Threonine 180.
• Immunogen TypePeptide-KLH
• Immunogen DescriptionPeptide sequence around phosphorylation site of threonine 180 (E-M-T(p)-G-Y ) derived from Human P38MAPK.
• Target NameP38 MAPK
• ModificationPhospho
• Alternative Names    MAPK2; MAPKAPK-2; MAPKAPK2
• Accession No. Swiss-Prot: Q16539
  NCBI Protein: NP _001306.1
• UniprotQ16539
• Gene ID1432;
• Concentration 1.0mg/ml
• Formulation Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Storage Store at -20°C for long term preservation (recommended). Store at 4°C for short term use.

• Predicted MW: 43kd
  Western blotting: 1:500~1:1000
  Immunohistochemistry: 1:50~1:100

Responds to activation by environmental stress, pro-inflammatory cytokines and lipopolysaccharide (LPS) by phosphorylating a number of transcription factors, such as ELK1 and ATF2 and several downstream kinases, such as MAPKAPK2 and MAPKAPK5. Plays a critical role in the production of some cytokines, for example IL-6. May play a role in stabilization of EPO mRNA during hypoxic stress. Isoform Mxi2 activation is stimulated by mitogens and oxidative stress and only poorly phosphorylates ELK1 and ATF2. Isoform Exip may play a role in the early onset of apoptosis.

Kim JE, et al. (2005) J Proteome Res Jul-Aug; 4(4): 1339-1346
Meng F, et al. (2005) Am J Physiol Cell Physiol May 25
Jin ZH, et al. (2005)Oncogene Mar 17; 24(12): 1973-1981

• Western blot analysis of extracts from HT29 cells untreated or treated with UV using P38 MAPK(Phospho-Thr180) Antibody #11252.
• Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using P38 MAPK(Phospho-Thr180) Antibody #11252(left) or the same antibody preincubated with blocking peptide(right).
• Western blot analysis of extracts from HL60 cells, treated with TNFa or calf intestinal phosphatase (CIP), using P38 MAPK (Phospho-Thr180) Antibody #11252.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting