- Product NameRecombinant Human Leptin protein(LEP) (Active)
- Brief DescriptionRecombinant Protein
- Purification>97% as determined by SDS-PAGE and HPLC.
- Biological ActivityFully biologically active when compared to standard. The ED50 as determined by a chemotaxis bioassay using human Leptin R transfected BaF3 murine proB cells is less than 2 ng/ml, corresponding to a specific activity of > 5.0 × 105 IU/mg.
- EndotoxinLess than 1 EU/μg of rHuLeptin as determined by LAL method.
- Immunogen DescriptionExpression Region:22-167aa
Sequence Info:Full Length of Mature Protein - Alternative Names Obese protein,
- Accession No. P41159
- UniprotP41159
- Gene ID3952;
- Calculated MW 16.0 kDa
- Tag Info NO-tagged
- Target Sequence VPIQKVQDDT KTLIKTIVTR INDISHTQSV SSKQKVTGLD FIPGLHPILT LSKMDQTLAV YQQILTSMPS RNVIQISNDL ENLRDLLHVL AFSKSCHLPW ASGLETLDSL GGVLEASGYS TEVVALSRLQ GSLQDMLWQL DLSPGC
- Formulation Lyophilized from a 0.2 μm filtered concentrated solution in 50 mm PB, pH 3.5, with 0.02 % Tween-20.
- Reconstitution We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.
- Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles. - 12 months from date of receipt, -20 to -70 °C as supplied. - 1 month, 2 to 8 °C under sterile conditions after reconstitution. - 3 months, -20 to -70 °C under sterile conditions after reconstitution.
