• Product NameAPH1 Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationAffinity chromatography purified via peptide column
• ApplicationsELISA WB
• Species ReactivityHu Ms
• Immunogen TypePeptide
• Immunogen DescriptionRaised against a 13 amino acid peptide from near the amino terminus of human APH1a.
• Target NameAPH1
• Alternative Names    Anterior pharynx defective 1, presenilin stabilization factor
• Accession No. Swiss-Prot:Q96BI3
  Gene ID:51107
• UniprotQ96BI3
• Gene ID51107;
• Concentration 1mg/ml
• Formulation Supplied in PBS containing 0.02% sodium azide.
• Storage Can be stored at -20˚C, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

APH1 was initially identified as a component of the Notch pathway in C. elegans. Along with nicastrin, PEN2, and presenilin-1 APH1 is an essential component of the gamma-secretase complex which cleave the amyloid precursor protein (APP) at what are known as the gamma- and epsilon-sites and can lead to the accumulation of the Amyloid beta peptide (Abeta) cleavage product that is associated with Alzheimer’s disease. APH1 exists in at least three distinct isoforms with APH1a as the principal isoform present in the gamma-secretase complex. Mice deficient in this isoform, but not the other two, were lethal at E10.5, with impaired vascular and neural development observed.

• Western blot analysis of APH1 in RAW264.7 cell lysate with APH1 antibody at (A) 0.5, (B) 1 and (C) 2 ug/mL.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting