• Product NameHistone H2A.X(Phospho-Ser139) Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationAntibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
• ApplicationsWB IF
• Species ReactivityHu
• SpecificityThe antibody detects endogenous level of Histone H2A.X only when phosphorylated at serine 139.
• Immunogen TypePeptide-KLH
• Immunogen DescriptionPeptide sequence around phosphorylation site of serine 139 (Q-A-S(p)-Q-E) derived from Human Histone H2A.X.
• Target NameHistone H2A.X
• ModificationPhospho
• Alternative Names    H2A.X; H2AFX; H2a/x; HIST5-2AX;
• Accession No. Swiss-Prot: P16104
  NCBI Protein: NP_002096.1
• UniprotP16104
• Gene ID3014;
• Concentration 1.0mg/ml
• Formulation Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Storage Store at -20°C for long term preservation (recommended). Store at 4°C for short term use.

• Predicted MW: 15kd
  Western blotting: 1:500~1:1000
  Immunofluorescence: 1:100~1:200

Variant histone H2A which replaces conventional H2A in a subset of nucleosomes. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. Required for checkpoint-mediated arrest of cell cycle progression in response to low doses of ionizing radiation and for efficient repair of DNA double strand breaks (DSBs) specifically when modified by C-terminal phosphorylation.

Yaneva M, et al. (2005) Nucleic Acids Res. 33(16): 5320-5330.
Tsukuda T, et al.(2006) Nature. Author manuscript; available in PMC 2006 March 6.

• Western blot analysis of extracts from HT29 cells untreated(lane 1) or treated with UV(lane 2) using Histone H2A.X(Phospho-Ser139) Antibody #11268.
• Immunofluorescence staining of methanol-fixed Hela cells using Histone H2A.X(Phospho-Ser139) Antibody #11268.
• Western blot analysis of extracts from 293 cells, treated with UV+serum or calf intestinal phosphatase (CIP), using Histone H2A.X (Phospho-Ser139) Antibody #11268.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting