• Product NamePKR(Phospho-Thr446) Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationAntibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
• ApplicationsWB
• Species ReactivityHuman,Mouse,Rat
• SpecificityThe antibody detects endogenous level of PKR only when phosphorylated at threonine 446.
• Immunogen TypePeptide-KLH
• Immunogen DescriptionPeptide sequence around phosphorylation site of threonine 446 (K-R-T(p)-R-S) derived from Human PKR.
• Target NamePKR
• ModificationPhospho
• Alternative Names    ADRB2; E2AK2; EIF2AK2; EIF2aK; PRKR
• Accession No. Swiss-Prot: P19525
  NCBI Protein: NP_001129123.1
• UniprotP19525
• Gene ID5610;
• Concentration 1.0mg/ml
• Formulation Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
• Storage Store at -20°C for long term preservation (recommended). Store at 4°C for short term use.

• WB 1:500 - 1:2000. 

Following activation by double-stranded RNA in the presence of ATP, the kinase becomes autophosphorylated and can catalyze the phosphorylation of the translation initiation factor EIF2S1, which leads to an inhibition of the initiation of protein synthesis. Double-stranded RNA is generated during the course of a viral infection.

Abujiang Pataer, et,al. (2002) Cancer Res; 62: 2239.
K. D. Ryman, et,al. (2005) J. Virol; 79: 1487 - 1499.
Susana Guerra, et,al. (2006) J. Biol. Chem; 281: 18734 - 18745.
 

• Western blot analysis of lysates from NIH/3T3 cells treated with IFN 2500U/ml 30', using PKR (Phospho-Thr446) Antibody. The lane on the right is blocked with the phospho peptide.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting