• Product NameIntegrin b3(Phospho-Tyr785) Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationAntibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
• ApplicationsWB
• Species ReactivityHu Ms Rt
• SpecificityThe antibody detects endogenous level of Integrin b3 only when phosphorylated at tyrosine 785.
• Immunogen TypePeptide-KLH
• Immunogen DescriptionPeptide sequence around phosphorylation site of tyrosine 785 (I-T-Y(p)-R-G) derived from Human Integrin b3.
• Target NameIntegrin b3
• ModificationPhospho
• Alternative Names    CD61 antigen; GP3A; GPIIIa; ITB3; Platelet membrane glycoprotein IIIa
• Accession No. Swiss-Prot: P05106
  NCBI Protein: NP _000203.2
• UniprotP05106
• Gene ID3690;
• Concentration 1.0mg/ml
• Formulation Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Storage Store at -20°C for long term preservation (recommended). Store at 4°C for short term use.

• Predicted MW: 110kd
  Western blotting: 1:500~1:1000
  

Integrin a-V/beta-3 is a receptor for cytotactin, fibronectin, laminin, matrix metalloproteinase-2, osteopontin, osteomodulin, prothrombin, thrombospondin, vitronectin and von Willebrand factor. Integrin a-IIb/beta-3 is a receptor for fibronectin, fibrinogen, plasminogen, prothrombin, thrombospondin and vitronectin. Integrins a-IIb/beta-3 and a-V/beta-3 recognize the sequence R-G-D in a wide array of ligands. Integrin a-IIb/beta-3 recognizes the sequence H-H-L-G-G-G-A-K-Q-A-G-D-V in fibrinogen gamma chain. Following activation integrin a-IIb/beta-3 brings about platelet/platelet interaction through binding of soluble fibrinogen. This step leads to rapid platelet aggregation which physically plugs ruptured endothelial surface. In case of HIV-1 infection, the interaction with extracellular viral Tat protein seems to enhance angiogenesis in Kaposi's sarcoma lesions.

Sujoy Bhattacharya, et al. (2006) Biochem J. August 1; 397(Pt 3): 437

• Western blot analysis of extracts from HUVEC cells using Integrin b3(Phospho-Tyr785) Antibody #11282 and the same antibody preincubated with blocking peptide .

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting