• Product NamePKR(Phospho-Thr451) Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationAntibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
• ApplicationsIHC
• Species ReactivityHu
• SpecificityThe antibody detects endogenous level of PKR only when phosphorylated at threonine 451.
• Immunogen TypePeptide-KLH
• Immunogen DescriptionPeptide sequence around phosphorylation site of threonine 451 (K-G-T(p)-L-R) derived from Human PKR.
• Target NamePKR
• ModificationPhospho
• Alternative Names    ADRB2; E2AK2; EIF2AK2; EIF2aK; PRKR
• Accession No. Swiss-Prot: P19525
  NCBI Protein: NP_001129123.1
• UniprotP19525
• Gene ID5610;
• Concentration 1.0mg/ml
• Formulation Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Storage Store at -20°C for long term preservation (recommended). Store at 4°C for short term use.

• Predicted MW: 68kd
  Immunohistochemistry: 1:50~1:100

Following activation by double-stranded RNA in the presence of ATP, the kinase becomes autophosphorylated and can catalyze the phosphorylation of the translation initiation factor EIF2S1, which leads to an inhibition of the initiation of protein synthesis. Double-stranded RNA is generated during the course of a viral infection.

Ingrid K. Ruf, et,al. (2005) J. Virol ; 79: 14562 - 14569.
Christy M. Hebner,et,al. (2006) J. Gen. Virol ; 87: 3183 - 3193.
Rika van Huizen, et,al. (2003) J. Biol. Chem ; 278: 15558 - 15564.

• Immunohistochemical analysis of paraffin-embedded human colon carcinoma tissue using PKR(Phospho-Thr451) Antibody #11290(left) or the same antibody preincubated with blocking peptide(right).

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting