• Product NamePKCd(Phospho-Ser645) Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationAntibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
• ApplicationsWB IHC
• Species ReactivityHu Rt
• SpecificityThe antibody detects endogenous level of PKCd only when phosphorylated at serine 645.
• Immunogen TypePeptide-KLH
• Immunogen DescriptionPeptide sequence around phosphorylation site of serine 645 (R-L-S(p)-Y-S) derived from Human PKCd.
• Target NamePKCd
• ModificationPhospho
• Alternative Names    KPCD; PKC-delta; PRKCD; kinase PKC-delta; nPKC-delta
• Accession No. Swiss-Prot: Q05655
  NCBI Protein: NP_006245.2
• UniprotQ05655
• Gene ID5580;
• Concentration 1.0mg/ml
• Formulation Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Storage Store at -20°C for long term preservation (recommended). Store at 4°C for short term use.

• Predicted MW: 78kd
  Western blotting: 1:500~1:1000
  Immunohistochemistry: 1:50~1:100
  

This is calcium-independent, phospholipid-dependent, serine- and threonine-specific enzyme. PKC is activated by diacylglycerol which in turn phosphorylates a range of cellular proteins. PKC also serves as the receptor for phorbol esters, a class of tumor promoters. May play a role in antigen-dependent control of B-cell function. Phosphorylates MUC1 in the C-terminal and regulates the interaction between MUC1 and beta-catenin.

Kei Sakamoto, et,al. (2003) Am J Physiol Endocrinol Metab ; 285: E1081 - E1088.
Ling Zhang, et,al. (2004) J. Biol. Chem ; 279: 28315 - 28319.
Kristof Van Kolen et,al. (2006) FEBS J ; 273: 1843 - 1854.
Martin Villalba, et,al. (2002) J. Cell Biol ; 157: 253.

• Western blot analysis of extracts from PC12 cells untreated or treated with PMA using PKCd(Phospho-Ser645) Antibody #11296.
• Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using PKCd(Phospho-Ser645) Antibody #11296(left) or the same antibody preincubated with blocking peptide(right).

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting