• Product NameKeratin 18(Phospho-Ser33) Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationAntibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
• ApplicationsWB IHC
• Species ReactivityHu
• SpecificityThe antibody detects endogenous level of Keratin 18 only when phosphorylated at serine 33.
• Immunogen TypePeptide-KLH
• Immunogen DescriptionPeptide sequence around phosphorylation site of serine 33 (A-A-S(p)-V-Y) derived from Human Keratin 18 (CK18).
• Target NameKeratin 18
• ModificationPhospho
• Alternative Names    CK 18; CK18; CYK18; Cytokeratin endo B; K18
• Accession No. Swiss-Prot: P05783
  NCBI Protein: NP_000215.1
• UniprotP05783
• Gene ID3875;
• Concentration 1.0mg/ml
• Formulation Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Storage Store at -20°C for long term preservation (recommended). Store at 4°C for short term use.

• Predicted MW: 46kd
  Western blotting: 1:500~1:1000
  Immunohistochemistry: 1:50~1:100
  

KRT18 encodes the type I intermediate filament chain keratin 18. Keratin 18, together with its filament partner keratin 8, are perhaps the most commonly found members of the intermediate filament gene family. They are expressed in single layer epithelial tissues of the body. utations in this gene have been linked to cryptogenic cirrhosis. Two transcript variants encoding the same protein have been found for this gene.

Ramaekers, F.C. and Bosman, F.T. (2004) J. Pathol. 204, 351-354.
Chang, L. and Goldman, R.D. (2004) Nat. Rev. Mol. Cell Biol. 5, 601-613.
Moll, R. et al. (1982) Cell 31, 11-24.

• Western blot analysis of extracts from HT29 cells untreated or treated with EGF using Keratin 18(Phospho-Ser33) Antibody #11306.
• Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using Keratin 18(Phospho-Ser33) Antibody #11306(left) or the same antibody preincubated with blocking peptide(right).

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting