• Product NameRSPO1 Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationAffinity chromatography purified via peptide column
• ApplicationsELISA WB IHC
• Species ReactivityHu Ms Rt
• Immunogen TypePeptide
• Immunogen DescriptionRaised against a 16 amino acid peptide from near the amino terminus of human RSPO1.
• Target NameRSPO1
• Alternative Names    R-spondin1, RSPO, CRISTIN3
• Accession No. Swiss-Prot:Q2MKA7
  Gene ID:284654
• UniprotQ2MKA7
• Gene ID284654;
• Concentration 1mg/ml
• Formulation Supplied in PBS containing 0.02% sodium azide.
• Storage Can be stored at -20˚C, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

RSPO1 is a member of a family of secreted growth factors that can operate through the canonical Wnt signaling pathway by stabilizing the intracellular beta-catenin, thereby regulating functions mediated by beta-catenin such as cell fate decisions and embryonic patterning. RSPO1 was recently identified through linkage analysis to be involved in sex determination and mammalian ovarian development. RSPO1 is thought to regulate cellular responsiveness to Wnt ligands by modulating the cell-surface expression of the Wnt co-receptor LRP6 by interfering with the DKK/Kremen-mediated internalization of LRP6 through an interaction with Kremen, resulting in increased LRP6 cell-surface levels. At least two isoforms of RSPO1 are known to exist. RSPO1 antibody will not cross-react with RSPO family members.

• Western blot analysis of RSPO1 in rat spleen tissue lysate with RSPO1 antibody at (A) 1 and (B) 2 ug/mL.
• Immunohistochemistry of RSPO1 in human spleen tissue with RSPO1 antibody at 2.5 ug/mL.
• Immunofluorescence of RSPO1 in human spleen tissue with RSPO1 antibody at 5 μg/ml.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting