• Product NameSHP-1(Phospho-Tyr536) Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
• ApplicationsWB IHC IF
• Species ReactivityHu Ms Rt
• SpecificityThe antibody detects endogenous level of SHP-1 only when phosphorylated at tyrosine 536.
• Immunogen TypePeptide-KLH
• Immunogen DescriptionPeptide sequence around phosphorylation site of tyrosine 536 (S-E-Y(p)-G-N) derived from Human SHP-1.
• Target NameSHP-1
• ModificationPhospho
• Alternative Names    70Z-SHP; HCP; HCPH; Hematopoietic cell protein-tyrosine phosphatase; PTN6
• Accession No. Swiss-Prot: P29350
  NCBI Protein: NP_002822.2
• UniprotP29350
• Gene ID5777;
• Concentration 1.0mg/ml
• Formulation Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
• Storage Store at -20°C for long term preservation (recommended). Store at 4°C for short term use.

• WB 1:500 - 1:2000. IHC 1:100 - 1:300 . IF 1:50-200

Acts downstream of various receptor and cytoplasmic protein tyrosine kinases to participate in the signal transduction from the cell surface to the nucleus.

Migone TS, et al. (1998) Proc Natl Acad Sci USA; 95(7): 3845-3850.
Timms JF, et al.(1998) Mol Cell Biol; 18(7): 3838-3850.
Kanagasundaram V, et al. (1999) Mol Cell Biol; 19(6): 4079-4092.
Hauck CR, et al. (1999) Infect Immun; 67(10): 5490-5494.

• Western Blot analysis of RAW264.7+EGF cells
• Western blot analysis of lysates from RAW264.7 cells treated with EGF 200ng/ml 5'. The lane on the right is blocked with the phospho peptide.
• Immunohistochemical analysis of paraffin-embedded Human breast cancer. Antibody was diluted at 1:100(4° overnight). High-pressure and temperature Tris-EDTA,pH8.0 was used for antigen retrieval. Negetive contrl (right) obtaned from antibody was pre-absorbed by immunogen peptide.
• Immunohistochemistry analysis of paraffin-embedded human breast carcinoma. The picture on the right is blocked with the phospho peptide.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting