• Product NameJMJD1B Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationAffinity chromatography purified via peptide column
• ApplicationsELISA WB IHC
• Species ReactivityHu Ms Rt
• SpecificityThis antibody will not cross-react with JMJD1A or JMJD1C.
• Immunogen TypePeptide
• Immunogen DescriptionRaised against a 20 amino acid peptide from near the center of human JMJD1B.
• Target NameJMJD1B
• Alternative Names    5qNCA, Lysine-specific demethylase 3B, KDM3B
• Accession No. Swiss-Prot:Q7LBC6
  Gene ID:51780
• UniprotQ7LBC6
• Gene ID51780;
• Concentration 1mg/ml
• Formulation Supplied in PBS containing 0.02% sodium azide.
• Storage Can be stored at -20˚C, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

The jumonji domain containing 1B protein (JMJD1B) was originally discovered as a candidate for the myeloid leukemia tumor suppressor gene. Besides CD34+ cells and acute myeloid leukemia (AML) cell lines, JMJD1B mRNA is highly expressed in liver, heart, kidney, skeletal muscle, and placenta tissues. The JMJD1B gene is localized to a region of chromosome 5q31, which is frequently deleted in myeloid leukemias and myelodysplasias and expression of JMJD1B in a del(5q) cell line results in suppression of clonogenic growth suggesting that JMJD1B may function as a tumor suppressor. In contrast, JMJD1B gene copy number and mRNA expression level was increased in several non-small cell lung cancers indicating that the role of JMJD1B in cancer formation and progression is more complex than originally postulated. At least three isoforms of JMJD1B are known to exist.

• Western blot analysis of JMJD1B in rat liver tissue lysate with JMJD1B antibody at (A) 1 and (B) 2 ug/mL.
• Immunohistochemistry of JMJD1B in rat liver tissue with JMJD1B antibody at 2.5 ug/mL.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting