P38 MAPK(Phospho-Thr180/Tyr182) antibody - SAB

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P38 MAPK(Phospho-Thr180/Tyr182) antibody #11581

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Catalog No.	Package Size	Price	Quantity
11581-1	50ul	$339.00
11581-2	100ul	$529.00

Availability:Yes

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Description

Product NameP38 MAPK(Phospho-Thr180/Tyr182) antibody
Host SpeciesRabbit
ClonalityPolyclonal
ApplicationsWB
Species ReactivityHu Ms Rt
SpecificityThe antibody detects endogenous level of P38 MAPK Protein only when phosphorylated at threonine 180/tyrosine 182.
Immunogen TypePeptide-KLH
Immunogen DescriptionPeptide sequence around phosphorylation site of threonine 180 or tyrosine 182 (E-M-T(p)-G-Y(p)-V-A) derived from Human P38 MAPK.
Target NameP38 MAPK
ModificationPhospho
Alternative Names RK, p38, CSBP, EXIP, Mxi2

Accession No. Swiss-Prot:Q16539
Gene ID:31
UniprotQ16539
Gene ID1432;
Concentration 1.0mg/ml

Application Details

Background

Serine/threonine kinase which acts as an essential component of the MAP kinase signal transduction pathway. MAPK14 is one of the four p38 MAPKs which play an important role in the cascades of cellular responses evoked by extracellular stimuli such as proinflammatory cytokines or physical stress leading to direct activation of transcription factors. Accordingly, p38 MAPKs phosphorylate a broad range of proteins and it has been estimated that they may have approximately 200 to 300 substrates each. Some of the targets are downstream kinases which are activated through phosphorylation and further phosphorylate additional targets. RPS6KA5/MSK1 and RPS6KA4/MSK2 can directly phosphorylate and activate transcription factors such as CREB1, ATF1, the NF-kappa-B isoform RELA/NFKB3, STAT1 and STAT3, but can also phosphorylate histone H3 and the nucleosomal protein HMGN1. RPS6KA5/MSK1 and RPS6KA4/MSK2 play important roles in the rapid induction of immediate-early genes in response to stress or mitogenic stimuli, either by inducing chromatin remodeling or by recruiting the transcription machinery. On the other hand, two other kinase targets, MAPKAPK2/MK2 and MAPKAPK3/MK3, participate in the control of gene expression mostly at the post-transcriptional level, by phosphorylating ZFP36 (tristetraprolin) and ELAVL1, and by regulating EEF2K, which is important for the elongation of mRNA during translation. MKNK1/MNK1 and MKNK2/MNK2, two other kinases activated by p38 MAPKs, regulate protein synthesis by phosphorylating the initiation factor EIF4E2. MAPK14 interacts also with casein kinase II, leading to its activation through autophosphorylation and further phosphorylation of TP53/p53. In the cytoplasm, the p38 MAPK pathway is an important regulator of protein turnover. For example, CFLAR is an inhibitor of TNF-induced apoptosis whose proteasome-mediated degradation is regulated by p38 MAPK phosphorylation. In a similar way, MAPK14 phosphorylates the ubiquitin ligase SIAH2, regulating its activity towards EGLN3. MAPK14 may also inhibit the lysosomal degradation pathway of autophagy by interfering with the intracellular trafficking of the transmembrane protein ATG9. Another function of MAPK14 is to regulate the endocytosis of membrane receptors by different mechanisms that impinge on the small GTPase RAB5A. In addition, clathrin-mediated EGFR internalization induced by inflammatory cytokines and UV irradiation depends on MAPK14-mediated phosphorylation of EGFR itself as well as of RAB5A effectors. Ectodomain shedding of transmembrane proteins is regulated by p38 MAPKs as well. In response to inflammatory stimuli, p38 MAPKs phosphorylate the membrane-associated metalloprotease ADAM17. Such phosphorylation is required for ADAM17-mediated ectodomain shedding of TGF-alpha family ligands, which results in the activation of EGFR signaling and cell proliferation. Another p38 MAPK substrate is FGFR1. FGFR1 can be translocated from the extracellular space into the cytosol and nucleus of target cells, and regulates processes such as rRNA synthesis and cell growth. FGFR1 translocation requires p38 MAPK activation. In the nucleus, many transcription factors are phosphorylated and activated by p38 MAPKs in response to different stimuli. Classical examples include ATF1, ATF2, ATF6, ELK1, PTPRH, DDIT3, TP53/p53 and MEF2C and MEF2A. The p38 MAPKs are emerging as important modulators of gene expression by regulating chromatin modifiers and remodelers. The promoters of several genes involved in the inflammatory response, such as IL6, IL8 and IL12B, display a p38 MAPK-dependent enrichment of histone H3 phosphorylation on 'Ser-10' (H3S10ph) in LPS-stimulated myeloid cells. This phosphorylation enhances the accessibility of the cryptic NF-kappa-B-binding sites marking promoters for increased NF-kappa-B recruitment. Phosphorylates CDC25B and CDC25C which is required for binding to 14-3-3 proteins and leads to initiation of a G2 delay after ultraviolet radiation. Phosphorylates TIAR following DNA damage, releasing TIAR from GADD45A mRNA and preventing mRNA degradation. The p38 MAPKs may also have kinase-independent roles, which are thought to be due to the binding to targets in the absence of phosphorylation. Protein O-Glc-N-acylation catalyzed by the OGT is regulated by MAPK14, and, although OGT does not seem to be phosphorylated by MAPK14, their interaction increases upon MAPK14 activation induced by glucose deprivation. This interaction may regulate OGT activity by recruiting it to specific targets such as neurofilament H, stimulating its O-Glc-N-acylation. Required in mid-fetal development for the growth of embryo-derived blood vessels in the labyrinth layer of the placenta. Also plays an essential role in developmental and stress-induced erythropoiesis, through regulation of EPO gene expression. Isoform MXI2 activation is stimulated by mitogens and oxidative stress and only poorly phosphorylates ELK1 and ATF2. Isoform EXIP may play a role in the early onset of apoptosis.

Published Papers

Corilagin ameliorates macrophages inflammation in atherosclerosis through TLR4-NFκB/MAPK pathwayIn HeliyonOn2023 Jun 13byDa Meng , Xin Deng et al..PMID:37383215, , (2023),
PMID: 37383215 CD155 Cooperates with PD-1/PD-L1 to Promote Proliferation of Esophageal Squamous Cancer Cells via PI3K/Akt and MAPK Signaling Pathways. In Cancers (Basel) on 2022 Nov 15 by Xiyang Tan, Jie Yang, et al..PMID:36428703, , (2022),
PMID: 36428703 Brevilin A Ameliorates Acute Lung Injury and Inflammation Through Inhibition of NF-κB Signaling via Targeting IKKα/β. In Front Pharmacol on 2022 Jun 14 by Lu Liu, Xian Chen,et al..PMID:35774606, , (2022),
PMID: 35774606 Role of 5-HT degradation in acute liver injury induced by carbon tetrachloride. In Eur J Pharmacol on 2021 Oct 5 by Yu-Xin Zhang, Chen Li,et al..PMID:34280394, , (2021),
PMID: 34280394 View All Published Papers Enhancement of anti-acne effect of Scutellaria baicalensis extract by fermentation with symbiotic fungus Penicillium decumbens. In J Biosci Bioeng on 2020 Nov by Xiaojing Zhu, Yue Mao, et al..PMID: 32747300, , (2020),
PMID: 32747300 miR-141-5p regulate ATF2 via effecting MAPK1/ERK2 signalIng to promote preeclampsia.Biomed Pharmacother. In Biomed Pharmacother on 2019 May 7 by Wang Y1, Cheng K, et al..PMID: 31075732, , (2019),
PMID: 31075732 HMGN2 regulates non-tuberculous mycobacteria survival via modulation of M1 macrophage polarization. In J Cell Mol Med on 2019 Dec by Wang X, Chen S, et al..PMID:31596045, , (2019),
PMID: 31596045 The protective effects of β-sitosterol and vermicularIn from Thamnolia vermicularis (Sw.) Ach. agaInst skIn agIng In vitro.In An Acad Bras Cienc on 2019 Dec 2 by Haiyuan YU, Shen X,et al..PMID:31800700, , (2019),
PMID: 31800700 Astragalus polysaccharides exerts anti-infective activity by inducing human cathelicidin antimicrobial peptide LL-37 in respiratory epithelial cells.In Phytother Res. On 2018 Aug by Zhao L, Tan S et al..PMID: 29672953, , (2018),
PMID: 29672953 Molecular mechanisms of somatostatin-mediated intestinal epithelial barrier function restoration by upregulating claudin-4 in mice with DSS-induced colitis.In Am J Physiol Cell Physiol. On 2018 Oct 1 by Cai L, Li X et al..PMID: 30020824, , (2018),
PMID: 30020824 Ajudecumin A from Ajuga ovalifolia var. calantha exhibits anti-inflammatory activity in lipopolysaccharide-activated RAW264.7 murine macrophages and animal models of acute inflammation.In Pharm Biol. On 2018 Dec by Zhang H, Ren QC et al..PMID: 31070535 , , (2018),
PMID: 31070535 Astragaloside IV Inhibits Isoprenaline?induced Cardiac Fibrosis by Targeting the Reactive Oxygen Species/Mitogen?activated Protein Kinase Signaling Axis.In Mol Med Rep on 2017 Apr by Hongliang Dai ,Guizhi Jia ,et al..PMID:28260010, , (2017),
PMID: 28260010 Ellagic Acid Inhibits RANKL-induced Osteoclast Differentiation by Suppressing the p38 MAP Kinase Pathway.In Arch Pharm Res on 2017 Jan by Mpho Rantlha , Travers Sagar,et al..PMID: 27384064, , (2017),
PMID: 27384064 Down-regulation of RPS9 inhibits osteosarcoma cell growth through inactivation of MAPK signaling pathway.In J Cancer on 2017 Aug 22 by Dong-Dong Cheng, Bin Zhu,et al..PMID: 28928861, , (2017),
PMID: 28928861 Altered Timing of Riboflavin and Ultraviolet Light Pathogen Inactivation Improves Platelet in Vitro Quality. In Transfusion on 2017 Aug by Peter Schubert, Brankica Culibrk,et al..PMID: 28500654, , (2017),
PMID: 28500654 Hepatic SATB1 induces paracrine activation of hepatic stellate cells and is upregulated by HBx.In Sci Rep.On 2016 Nov 24 by Gong J, Tu W et al..PMID:27883059, , (2016),
PMID: 27883059 Potassium supplementation inhibits IL-17A production induced by salt loading in human T lymphocytes via p38/MAPK-SGK1 pathway.In Exp Mol Pathol.On 2016 Jun by Wen W, Wan Z et al..PMID:27020669, , (2016),
PMID: 27020669 The activation of p38 MAPK limits the abnormal proliferation of vascular smooth muscle cells induced by high sodium concentrations.In Int J Mol Med On 2016 Jan by Yan Wu , Juan Zhou et al..PMID:26530729, , (2016),
PMID: 26530729 The Effects of Xiangqing Anodyne Spray on treating acute soft-Tissue injury mainly depend on suppressing activations of AKT and p38 pathways.In Evid Based Complement Alternat Med on 2016 by Shudong Wang , Tao Li et al..PMID:27190541, , (2016),
PMID: 27190541 PiHOG1, a stress regulator MAP kinase from the root endophyte fungus Piriformospora indica, confers salinity stress tolerance in rice plants. In Sci Rep on 2016 Nov 16 by Abhimanyu Jogawat, Jyothilakshmi Vadassery, et al..PMID:27849025, , (2016),
PMID: 27849025 CPU-12, a novel synthesized oxazolo [5, 4-d] pyrimidine derivative, showed superior anti-angiogenic activity.In J Pharmacol Sci on 2015 Sep by Jiping Liu , Ya-Hui Deng et al..PMID: 26154846, , (2015),
PMID: 26154846 Piperine alleviates osteoclast formation through the p38/c-Fos/NFATc1 signaling axis.In Biofactors on Nov-Dec 2015 by Vishwa Deepak , Marlena C Kruger et al..PMID: 26627060, , (2015),
PMID: 26627060 Cannabinoid receptor agonist WIN55,212-2 and fatty acid amide hydrolase inhibitor URB597 suppress chronic cerebral hypoperfusion-induced neuronal apoptosis by inhibiting c-Jun N-terminal kinase signaling.In Neuroscience 2015 Aug 20 by S-H Su , Y-F Wu et al..PMID:25795598, , (2015),
PMID: 25795598 Quercetin regulates the sestrin 2-AMPK-p38 MAPK signaling pathway and induces apoptosis by increasing the generation of intracellular ROS in a p53-independent manner.In Int J Mol Med on 2014 Apr by Guen Tae Kim, Se Hee Lee et al..PMID:24535669, , (2014),
PMID: 24535669 Regulation of T lymphocyte activation by microRNA-21.In Mol Immunol on 2014 Jun by Lu Wang , Liangqiang He et al..PMID: 24631982, , (2014),
PMID: 24631982 Insights in dynamic kinome reprogramming as a consequence of MEK inhibition in MLL-rearranged AML.In Leukemia on 2014 Mar by K R Kampen, A Ter Elst et al..PMID:24240200, , (2014),
PMID: 24240200 Toosendanin Induces Apoptosis Through Suppression of JNK Signaling Pathway in HL-60 Cells.In Toxicol In Vitro on 2013 Feb by Jianming Ju, Zhichao Qi,et al..PMID:23111283, , (2013),
PMID: 23111283 New roles of the fission yeast eIF2α kinases Hri1 and Gcn2 in response to nutritional stress. In J Cell Sci on 2013 Jul 15 by Ruth Martn, Juan Jos Berlanga, et al..PMID: 23687372, , (2013),
PMID: 23687372 The Anti-Inflammatory Mechanism of Heme oxygenase-1 Induced by Hemin in Primary Rat Alveolar Macrophages.In Inflammation on 2012 Jun by Chen Hualin, Xu Wenli, et al..PMID:22160840, , (2012),
PMID: 22160840 Cardamonin Protects Septic Mice From Acute Lung Injury by Preventing Endothelial Barrier Dysfunction.In J Biochem Mol Toxicol on 2012 Jul by Zhifeng Wei, Jian Yang,et al..PMID:22696397, , (2012),
PMID: 22696397 EphrinBs/EphBs signaling is involved in modulation of spinal nociceptive processing through a mitogen-activated protein kinases-dependent mechanism. In Anesthesiology on 2010 May by Jia-Ping Ruan, Hong-Xing Zhang, et al..PMID: 20395829, , (2010),
PMID: 20395829 Lidocaine Attenuates Proinflammatory Cytokine Production Induced by Extracellular Adenosine Triphosphate in Cultured Rat Microglia.In Anesth Analg on 2010 Sep by Diansan Su, Yang Gu,et al..PMID:20686009, , (2010),
PMID: 20686009 EphrinBs/EphBs signaling is involved in modulation of spinal nociceptive processing through a mitogen-activated protein kinases-dependent mechanism. In Anesthesiology on 2010 May by Jia-Ping Ruan, Hong-Xing Zhang, et al..PMID: 20395829, , (2010),
PMID: 20395829 Disruption of xCT inhibits cancer cell metastasis via the caveolin-1/β-catenin pathway. In Oncogene on 2009 Jan 29 by R-S Chen, Y-M Song, et al..PMID: 19015640, , (2009),
PMID: 19015640 Attenuating Phosphorylation of p38 MAPK in the Activated Microglia: A New Mechanism for Intrathecal Lidocaine Reversing Tactile Allodynia Following Chronic Constriction Injury in Rats.In Neurosci Lett on 2008 Jan 31 by Yi-Wen Gu, Dian-San Su,et al..PMID:18191894, , (2008),
PMID: 18191894 Please let us know if you have published research using #11581 so that we can cite your reference.

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P38 MAPK(Phospho-Thr180/Tyr182...(11581)

NOTE

Application

WBWestern Blotting

IHCImmunohistochemistry

IFImmunofluorescence

ICCImmunocytochemistry

FCFlow Cytometry

IPImmunoprecipitation

EELISA

DBDot Blotting

ChIPChromatin Immunoprecipitation

GICAGold Immunochromatography Assay

NCNegative Control

Species Reactivity

HuHuman

MsMouse

RtRat

Dm Drosophila melanogaster

C Caenorhabditis elegans

MkMonkey

RbRabbit

B Bovine

D Dog

PPig

HmHamster

ChHm Chinese Hamster

ChkChicken

ShpSheep