• Product NameSmad2(Phospho-Ser467) Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationAntibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
• ApplicationsWB IHC
• Species ReactivityHu Ms Rt
• SpecificityThe antibody detects endogenous level of Smad2 only when phosphorylated at serine 467.
• Immunogen TypePeptide-KLH
• Immunogen DescriptionPeptide sequence around phosphorylation site of serine 467 (C-S-S-M-S(p)) derived from Human Smad2.
• Target NameSmad2
• ModificationPhospho
• Alternative Names    JV18-1; MADH2; MADR2; Mad-related protein 2; Mothers against DPP homolog 2
• Accession No. Swiss-Prot: Q15796
  NCBI Protein: NP _001003652.1
• UniprotQ15796
• Gene ID4087;
• Concentration 1.0mg/ml
• Formulation Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Storage Store at -20°C for long term preservation (recommended). Store at 4°C for short term use.

• Predicted MW: 60kd
  Western blotting: 1:500~1:1000

  IHC 1:50-1:200

Transcriptional modulator activated by TGF-beta and activin type 1 receptor kinase. SMAD2 is a receptor-regulated SMAD (R-SMAD). May act as a tumor suppressor in colorectal carcinoma.

Sang Gyun Kim et al. (2004) Mol Biol Cell. February; 15(2): 420

• Western blot analysis of extracts from Hela cells untreated or treated with PMA using Smad2(Phospho-Ser467) Antibody #11322.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting