• Product NameNALP3 Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationAffinity chromatography purified via peptide column
• ApplicationsELISA WB IF
• Species ReactivityHu Ms
• Immunogen TypePeptide
• Immunogen DescriptionRaised against a 16 amino acid peptide from near the amino terminus of human NALP3.
• Target NameNALP3
• Alternative Names    LRR and PYD domains-containing protein 3, CIAS1, FCAS, Cryopyrin
• Accession No. Swiss-Prot:Q96P20
  Gene ID:114548
• UniprotQ96P20
• Gene ID114548;
• Concentration 1mg/ml
• Formulation Supplied in PBS containing 0.02% sodium azide.
• Storage Can be stored at -20˚C, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

NALP3, a member of Nod-like receptors, has a crucial role in inflammation and immunity and may be a proximal sensor of cellular stress and danger signals. NALP3 forms a caspase-1 activating molecular complex termed the inflammasome. The inflammasome allows activation of IL-1β, the key player of the inflammation and fever. NALP3 gene encodes a pyrin like protein which contains a pyrin domain, a nucleotide binding site (NBS) domain, and a leucine rich repeat (LRR) motif. NALP3 protein interacts with apoptosis associated speck like protein containing a CARD and may function as an inducer of apoptosis and an activator of NF-κB signaling. Defects in NALP3 have been associated with familial cold autoinflammatory syndrome (FCAS), Muckle-Wells syndrome (MWS), chronic infantile neurological cutaneous and articular (CINCA) syndrome, and neonatal-onset multisystem inflammatory disease (NOMID).

• Western blot analysis of NALP3 in K562 cell lysate with NALP3 antibody at 1 ug/mL (A) in the absence and (B) in the presence of blocking peptide.
• Immunofluorescence of NALP3 in K562 cells with NALP3 antibody at 20 ug/mL.
• Western blot analysis of NALP3 in K562 cell lysate with NALP3 antibody at 1 μg/mL (A) in the absence and (B) in the presence of blocking peptide.
• Immunocytochemistry of NALP3 in K562 cells with NALP3 antibody at 2 μg/mL.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting