• Product NameSmad3(Phospho-Ser425) Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationAntibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
• ApplicationsWB IHC
• Species ReactivityHu Ms Rt
• SpecificityThe antibody detects endogenous level of Smad3 only when phosphorylated at serine 425.
• Immunogen TypePeptide-KLH
• Immunogen DescriptionPeptide sequence around phosphorylation site of serine 425 (C-S-S-V-S(p)) derived from Human Smad3.
• Target NameSmad3
• ModificationPhospho
• Alternative Names    JV15-2; MAD-3; MADH3; Mad3; Mothers against DPP homolog 3
• Accession No. Swiss-Prot: P84022
  NCBI Protein: NP_001138574.1
• UniprotP84022
• Gene ID4088;
• Concentration 1.0mg/ml
• Formulation Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Storage Store at -20°C for long term preservation (recommended). Store at 4°C for short term use.

• Predicted MW: 52kd
  Western blotting: 1:500~1:1000
  Immunohistochemistry: 1:50~1:100
  

Smad3 encoded by this gene belongs to the SMAD, a family of proteins similar to the gene products of the Drosophila gene 'mothers against decapentaplegic' (Mad) and the C. elegans gene Sma. SMAD proteins are signal transducers and transcriptional modulators that mediate multiple signaling pathways. This protein functions as a transcriptional modulator activated by transforming growth factor-beta and is thought to play a role in the regulation of carcinogenesis.

Shi W, et al. J Cell Sci. 2007 Apr 1;120(Pt 7):1216-24
Seong HA, et al. J Biol Chem. 2007 Apr 20;282(16):12272-89
Wordinger RJ, et al. Invest Ophthalmol Vis Sci. 2007 Mar;48(3):1191-200
LeClair RJ, et al. Circ Res. 2007 Mar 30;100(6):826-33

• Western blot analysis of extracts from Hela cells untreated or treated with EGF using Smad3(Phospho-Ser425) Antibody #11325.
• Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using Smad3(Phospho-Ser425) Antibody #11325(left) or the same antibody preincubated with blocking peptide(right).

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting