• Product NameTrkA(Phospho-Tyr791) Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationAntibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
• ApplicationsWB IF
• Species ReactivityHu Ms Rt
• SpecificityThe antibody detects endogenous level of TrkA only when phosphorylated at tyrosine 791.
• Immunogen TypePeptide-KLH
• Immunogen DescriptionPeptide sequence around phosphorylation site of tyrosine791 (P-V-Y(p)-L-D) derived from Human TrkA.
• Target NameTrkA
• ModificationPhospho
• Alternative Names    High affinity nerve growth factor receptor precursor; NTRK1; Slow nerve growth factor receptor; TRK; TRK1 transforming tyrosine kinase protein
• Accession No. Swiss-Prot: P04629
  NCBI Protein: NP_001007793.1
• UniprotP04629
• Gene ID4914;
• Concentration 1.0mg/ml
• Formulation Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Storage Store at -20°C for long term preservation (recommended). Store at 4°C for short term use.

• Predicted MW: 140kd
  Western blotting: 1:500~1:1000
  Immunofluorescence: 1:100~1:200

Required for high-affinity binding to nerve growth factor (NGF), neurotrophin-3 and neurotrophin-4/5 but not brain-derived neurotrophic factor (BDNF). Known substrates for the Trk receptors are SHC1, PI 3-kinase, and PLC-gamma-1. Has a crucial role in the development and function of the nociceptive reception system as well as establishment of thermal regulation via sweating. Activates ERK1 by either SHC1- or PLC-gamma-1-dependent signaling pathway.

Wiese S, et al. Proc Natl Acad Sci U S A. 2007 Oct 23; 104(43):17210-5.
Valdez G, et al. Proc Natl Acad Sci U S A. 2007 Jul 24;104(30):12270-5
Inoue K, et al. J Biol Chem. 2007 Aug 17;282(33):24175-84

• Western blot analysis of extracts from Rat and Mouse brain tissue using TrkA(Phospho-Ser791) Antibody #11326.
• Immunofluorescence staining of methanol-fixed Hela cells using TrkA(Phospho-Ser791) Antibody #11326.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting