• Product NameTCF3 Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationAffinity chromatography purified via peptide column
• ApplicationsELISA WB IHC
• Species ReactivityHu Ms Rt
• Immunogen TypePeptide
• Immunogen DescriptionRaised against a 17 amino acid peptide near the amino terminus of human TCF3.
• Target NameTCF3
• Alternative Names    Immunoglobulin transcription factor 1, transcription factor 3, E12, E2A, ITF1, E47
• Accession No. Swiss-Prot:P15923
  Gene ID:6929
• UniprotP15923
• Gene ID6929;
• Concentration 1mg/ml
• Formulation Supplied in PBS containing 0.02% sodium azide.
• Storage Can be stored at -20˚C, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

The TCF3 gene, also called E2A, encodes two basic helix-loop-helix (bHLH) transcription factors, E12 and E47, through alternative splicing. These transcription factors are involved in mediating canonical Wnt signaling, which is very important in a diverse array of cellular functions such as stem cell proliferation, self-renewal, activation, fate determination, differentiation and aging and senescence. They bind beta-catenin and can act as transcriptional activators or repressors for Wnt target genes, and have been shown to regulate specific target genes during CNS development downstream of Wnt signaling. TCF3/Lef complexes are also known to play key roles in controlling cell fate lineages in multipotent skin stem cells.

• Western blot analysis of TCF3 in Human brain tissue lysate with TCF3 antibody at 1 ug/mL in (A) the absence and (B) presence of peptide blocking.
• Immunohistochemistry of TCF3 in rat liver tissue with TCF3 antibody at 5 ug/mL.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting