• Product Namecofilin1(phospho-Tyr139) Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationAntibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
• ApplicationsIHC
• Species ReactivityHu Ms Rt
• SpecificityThe antibody detects endogenous level of cofilin1 only when phosphorylated at tyrosine 139.
• Immunogen TypePeptide-KLH
• Immunogen DescriptionPeptide sequence around phosphorylation site of tyrosine 139 (N-C-Y(p)-E-E) derived from Human cofilin.
• Target Namecofilin1
• ModificationPhospho
• Alternative Names    CFL; CFL1;
• Accession No. Swiss-Prot: P23528
  NCBI Protein: NP_005498.1
• UniprotP23528
• Gene ID1072;
• Concentration 1.0mg/ml
• Formulation Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Storage Store at -20°C for long term preservation (recommended). Store at 4°C for short term use.

• Predicted MW: 19kd
  Immunohistochemistry: 1:50~1:100
  

Controls reversibly actin polymerization and depolymerization in a pH-sensitive manner. It has the ability to bind G- and F-actin in a 1:1 ratio of cofilin to actin. It is the major component of intranuclear and cytoplasmic actin rods.

Carlier, M. et al. (1999) J. Biol. Chem. 274, 33827-33830.
Arber, S. et al. (1998) Nature 393, 805-809.
Yang, N. et al. (1998) Nature 393, 809-812.

• Immunohistochemical analysis of paraffin-embedded human lung carcinoma tissue using cofilin1(Phospho-Tyr139) Antibody #11506(left) or the same antibody preincubated with blocking peptide(right).

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting