• Product NameCCDC69 Antibody
• ClonalityPolyclonal
• PurificationAffinity chromatography purified via peptide column
• ApplicationsELISA,WB,IHC-P,IF
• Species ReactivityHu Ms Rt
• Immunogen TypePeptide
• Immunogen DescriptionRaised against an 18 amino acid synthetic peptide from near the center of human CCDC69.
• Target NameCCDC69
• Alternative Names    Coiled coil domain-containing 69
• Accession No. Swiss-Prot:A6NI79
  Gene ID:26112
• UniprotA6NI79
• Gene ID26112;
• Calculated MW Predicted: 35kD Observed: 35 kD
• Concentration 1mg/ml
• Formulation Supplied in PBS containing 0.02% sodium azide.
• Storage Stored at 4℃ for three months and -20℃, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

The coiled-coil domain is a common protein motif that is often involved in protein oligomerization and is found in proteins such as transcription factors and intermediate filaments. The CCDC47 gene maps to chromosome 5 at 5q33.1. Little is known about this protein.

• WB Validation in Human Cell Lines Loading: 15 ug of lysate. Antibodies: CCDC69, 1 ug/mL , 1 h incubation at RT in 8% NFDM/TBST. Secondary: Goat Anti-Rabbit IgG HRP conjugate at 1:10,000 dilution.
• Western Blot Validation in Mouse Pancreas Tissue Lysate Loading: 15 ug of lysates per lane. Antibodies: CCDC69, 1 ug/mL, 1h incubation at RT in 8% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10,000 dilution.
• Western Blot Validation in Rat Pancreas Lysate Loading: 15 ug of lysates per lane. Antibodies: CCDC69, 1 u g/mL, 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10,000 dilution.
• Immunohistochemistry of CCDC69 in human lung tissue with CCDC69 antibody at 5 ug/mL.
• Immunofluorescence of CCDC69 in human lung tissue with CCDC69 antibody at 20 ug/mL.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting