• Product NameMGMT Rabbit mAb
• Host SpeciesRecombinant Rabbit
• ClonalityMonoclonal antibody
• Clone No.S01-8B1
• IsotypeRabbit IgG
• PurificationAffinity Purified
• ApplicationsWB IHC
• Species ReactivityHuman
• Immunogen DescriptionA synthetic peptide of human MGMT
• ConjugatesUnconjugated
• ModificationUnmodification
• Alternative Names    Methylated-DNA--protein-cysteine methyltransferase
• Accession No. Swiss-Prot:P16455
  GeneID:4255
• UniprotP16455
• Gene ID4255
• Calculated MW Calculated MW: 22 kDa; Observed MW: 22 kDa
• Concentration 0.3 mg/ml
• Formulation 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA
• Storage Store at 4˚C short term. Aliquot and store at -20˚C long term. Avoid freeze/thaw cycles.

• WB: 1/1000-1/5000; IHC: 1/50-1/200

Involved in the cellular defense against the biological effects of O6-methylguanine (O6-MeG) and O4-methylthymine (O4-MeT) in DNA. Repairs the methylated nucleobase in DNA by stoichiometrically transferring the methyl group to a cysteine residue in the enzyme. This is a suicide reaction: the enzyme is irreversibly inactivated.

• Western blot detection of MGMT in Jurkat cell lysates using MGMT Rabbit mAb(1:1000 diluted).Predicted band size:22kDa.Observed band size:22kDa.
• Immunohistochemistry of MGMT in paraffin-embedded Human colon cancer tissue using MGMT Rabbit mAb at dilution 1/100

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting