• Product NameUFM1 Rabbit mAb
• Host SpeciesRecombinant Rabbit
• ClonalityMonoclonal antibody
• Clone No.S02-5G1
• IsotypeRabbit IgG
• PurificationAffinity Purified
• ApplicationsWB IHC
• Species ReactivityHuman,Mouse,Rat
• Immunogen DescriptionA synthetic peptide of human UFM1
• ConjugatesUnconjugated
• ModificationUnmodification
• Alternative Names    Ubiquitin-fold modifier 1; C13orf20
• Accession No. Swiss-Prot:P61960
  GeneID:51569
• UniprotP61960
• Gene ID51569
• Calculated MW Calculated MW: 9 kDa; Observed MW: 9 kDa
• Concentration 0.3 mg/ml
• Formulation 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA
• Storage Store at 4˚C short term. Aliquot and store at -20˚C long term. Avoid freeze/thaw cycles.

• WB: 1/1000; IHC: 1/50-1/200;

Ubiquitin-like modifier which can be covalently attached via an isopeptide bond to lysine residues of substrate proteins as a monomer or a lysine-linked polymer (PubMed:15071506, PubMed:20018847, PubMed:29868776, PubMed:27653677). The so-called ufmylation, requires the UFM1-activating E1 enzyme UBA5, the UFM1-conjugating E2 enzyme UFC1, and the UFM1-ligase E3 enzyme UFL1 (PubMed:15071506, PubMed:20018847, PubMed:29868776, PubMed:27653677). Ufmylation is involved in reticulophagy (also called ER-phagy) induced in response to endoplasmic reticulum stress (PubMed:32160526). Ufmylation of TRIP4 regulates nuclear receptors-mediated transcription (PubMed:25219498).

• Western blot detection of UFM1 in Jurkat cell lysates using UFM1 Rabbit mAb(1:1000 diluted).Predicted band size:9kDa.Observed band size:9kDa.
• Immunohistochemistry of UFM1 in paraffin-embedded human lung cancer tissue using UFM1 Rabbit mAb at dilution 1/100

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting