• Product NamePRPF31 Rabbit mAb
• Host SpeciesRecombinant Rabbit
• ClonalityMonoclonal antibody
• Clone No.S01-7F1
• IsotypeRabbit IgG
• PurificationAffinity Purified
• ApplicationsWB IF
• Species ReactivityHuman,Rat
• Immunogen DescriptionA synthetic peptide of human PRPF31
• ConjugatesUnconjugated
• ModificationUnmodification
• Alternative Names    Protein 61K;hPrp31;U4/U6 snRNP 61 kDa protein
• Accession No. Swiss-Prot:Q8WWY3
  GeneID:26121
• UniprotQ8WWY3
• Gene ID26121
• Calculated MW Calculated MW: 56 kDa; Observed MW: 56 kDa
• Concentration 0.3 mg/ml
• Formulation 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA
• Storage Store at 4˚C short term. Aliquot and store at -20˚C long term. Avoid freeze/thaw cycles.

• WB: 1/1000-1/5000; ICC/IF: 1/50;

Swiss-Prot Acc.Q8WWY3.Involved in pre-mRNA splicing as component of the spliceosome (PubMed:11867543, PubMed:28781166). Required for the assembly of the U4/U5/U6 tri-snRNP complex, one of the building blocks of the spliceosome (PubMed:11867543).

• Western blot detection of PRPF31 in C2C12,PC3 cell lysates using PRPF31 Rabbit mAb(1:1000 diluted).Predicted band size:56kDa.Observed band size:56kDa.
• Immunofluorescence of PRPF31 (green) in Hela using PRPF31 Rabbit mAb at dilution 1/50, and DAPI(blue)

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting