• Product NameSHP2 Rabbit mAb
• Host SpeciesRecombinant Rabbit
• ClonalityMonoclonal antibody
• Clone No.S08-3G2
• IsotypeRabbit IgG
• PurificationAffinity Purified
• ApplicationsWB IHC
• Species ReactivityHuman
• Immunogen DescriptionA synthetic peptide of human SHP2
• ConjugatesUnconjugated
• ModificationUnmodification
• Alternative Names    CFC, NS1, SHP2, BPTP3, PTP2C, PTP-1D, SH-PTP2, SH-PTP
• Accession No. Swiss-Prot:Q06124
  GeneID:5781
• UniprotQ06124
• Gene ID5781
• Calculated MW Calculated MW: 68 kDa; Observed MW: 68 kDa
• Concentration 0.3 mg/ml
• Formulation 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA
• Storage Store at 4˚C short term. Aliquot and store at -20˚C long term. Avoid freeze/thaw cycles.

• WB: 1/2000; IHC: 1/20;

Swiss-Prot Acc.Q06124.Acts downstream of various receptor and cytoplasmic protein tyrosine kinases to participate in the signal transduction from the cell surface to the nucleus (PubMed:10655584, PubMed:18559669, PubMed:18829466, PubMed:26742426, PubMed:28074573). Positively regulates MAPK signal transduction pathway (PubMed:28074573). Dephosphorylates GAB1, ARHGAP35 and EGFR (PubMed:28074573). Dephosphorylates ROCK2 at 'Tyr-722' resulting in stimulation of its RhoA binding activity (PubMed:18559669). Dephosphorylates CDC73 (PubMed:26742426). Dephosphorylates SOX9 on tyrosine residues, leading to inactivate SOX9 and promote ossification (By similarity).

• Western blot detection of SHP2 in Hela,293 cell lysates using SHP2 Rabbit mAb(1:1000 diluted).Predicted band size:68kDa.Observed band size:68kDa.
• Immunohistochemistry of SHP2 in paraffin-embedded Human breast cancer tissue using SHP2 Rabbit mAb at dilution 1/50

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting