• Product NamemtTFA Rabbit mAb
• Host SpeciesRecombinant Rabbit
• ClonalityMonoclonal antibody
• Clone No.S08-3E2
• IsotypeRabbit IgG
• PurificationAffinity Purified
• ApplicationsWB IHC IF
• Species ReactivityHuman
• Immunogen DescriptionA synthetic peptide of human mtTFA
• ConjugatesUnconjugated
• ModificationUnmodification
• Alternative Names    MtTF1; mtTFA; TCF6; TCF6L1; TCF6L2; TCF6L3; TFAM; Transcription factor 6;
• Accession No. Swiss-Prot:Q00059
  GeneID:7019
• UniprotQ00059
• Gene ID7019
• Calculated MW Calculated MW: 29 kDa; Observed MW: 24 kDa
• Concentration 0.3 mg/ml
• Formulation 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA
• Storage Store at 4˚C short term. Aliquot and store at -20˚C long term. Avoid freeze/thaw cycles.

• WB: 1/2000-1/10000; IHC: 1/50; ICC/IF: 1/20-1/50;

Swiss-Prot Acc.Q00059.Binds to the mitochondrial light strand promoter and functions in mitochondrial transcription regulation (PubMed:29445193, PubMed:32183942). Component of the mitochondrial transcription initiation complex, composed at least of TFB2M, TFAM and POLRMT that is required for basal transcription of mitochondrial DNA (PubMed:29149603). In this complex, TFAM recruits POLRMT to a specific promoter whereas TFB2M induces structural changes in POLRMT to enable promoter opening and trapping of the DNA non-template strand (PubMed:20410300). Required for accurate and efficient promoter recognition by the mitochondrial RNA polymerase (PubMed:22037172). Promotes transcription initiation from the HSP1 and the light strand promoter by binding immediately upstream of transcriptional start sites (PubMed:22037172). Is able to unwind DNA (PubMed:22037172).

• Western blot detection of mtTFA in K562,Hela cell lysates using mtTFA Rabbit mAb(1:1000 diluted).Predicted band size:29kDa.Observed band size:24kDa.
• Immunofluorescence of mtTFA/TFAM(green) in Hela cells using mtTFA/TFAM Rabbit mAb at dilution 1/200, and DAPI(blue)
• Immunohistochemistry of mtTFA in paraffin-embedded Human breast cancer tissue using mtTFA Rabbit mAb at dilution 1/1

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting