• Product NameAnterior Gradient 2 Rabbit mAb
• Host SpeciesRecombinant Rabbit
• ClonalityMonoclonal antibody
• Clone No.S04-1H6
• IsotypeRabbit IgG
• PurificationAffinity Purified
• ApplicationsWB IHC IF
• Species ReactivityHuman, Rat
• Immunogen DescriptionA synthetic peptide of human Anterior Gradient 2
• ConjugatesUnconjugated
• ModificationUnmodification
• Alternative Names    Anterior gradient protein 2 homolog; AG-2; hAG-2; HPC8; Secreted cement gland protein XAG-2 homolog; AGR2; AG2; GOB4; XAG2;
• Accession No. Swiss-Prot:O95994
  GeneID:10551
• UniprotO95994
• Gene ID10551
• Calculated MW Calculated MW: 20 kDa; Observed MW: 18 kDa
• Concentration 0.3 mg/ml
• Formulation 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA
• Storage Store at 4˚C short term. Aliquot and store at -20˚C long term. Avoid freeze/thaw cycles.

• WB: 1/2000; IHC: 1/50; ICC/IF: 1/50;

Swiss-Prot Acc.O95994.Required for MUC2 post-transcriptional synthesis and secretion. May play a role in the production of mucus by intestinal cells (By similarity). Proto-oncogene that may play a role in cell migration, cell differentiation and cell growth. Promotes cell adhesion (PubMed:23274113).

• Western blot detection of Anterior Gradient 2 in K562,C6 cell lysates using Anterior Gradient 2 Rabbit mAb(1:1000 diluted).Predicted band size:20kDa.Observed band size:18kDa.
• Immunofluorescence of Anterior Gradient 2 (green) in MCF-7 using Anterior Gradient 2 antibody at dilution 1/2, and DAPI(blue)
• Immunohistochemistry of Anterior Gradient 2 in paraffin-embedded colon using Anterior Gradient 2 Rabbit mAb at dilution 1/100

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting