• Product NameCaspase 3 Rabbit mAb
• Host SpeciesRecombinant Rabbit
• ClonalityMonoclonal antibody
• Clone No.S02-7C2
• IsotypeRabbit IgG
• PurificationAffinity Purified
• ApplicationsWB IHC
• Species ReactivityHuman
• Immunogen DescriptionA synthetic peptide of human Caspase-3
• ConjugatesUnconjugated
• ModificationUnmodification
• Alternative Names    Caspase-3, CASP-3, Apopain, Cysteine protease CPP32, CPP-32, Protein Yama, SREBP cleavage activity 1, SCA-1, Caspase-3 subunit p17, Caspase-3 subunit p12, CASP3, CPP32.
• Accession No. Swiss-Prot:P42574
  GeneID:836
• UniprotP42574
• Gene ID836
• Calculated MW Calculated MW: 32 kDa; Observed MW: 32 kDa
• Concentration 0.3 mg/ml
• Formulation 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA
• Storage Store at 4˚C short term. Aliquot and store at -20˚C long term. Avoid freeze/thaw cycles.

• WB: 1/1000; IHC: 1/20;

Involved in the activation cascade of caspases responsible for apoptosis execution (PubMed:7596430). At the onset of apoptosis it proteolytically cleaves poly(ADP-ribose) polymerase (PARP) at a '216-Asp-|-Gly-217' bond (PubMed:7774019). Cleaves and activates sterol regulatory element binding proteins (SREBPs) between the basic helix-loop-helix leucine zipper domain and the membrane attachment domain. Cleaves and activates caspase-6, -7 and -9 (PubMed:7596430). Involved in the cleavage of huntingtin (PubMed:8696339).

• Western blot detection of Caspase-3 in K562,Hela cell lysates using Caspase-3 Rabbit mAb(1:1000 diluted).Predicted band size:32kDa.Observed band size:32kDa.
• Immunohistochemistry of Caspase-3 in paraffin-embedded Human lung cancer tissue using Caspase-3 Rabbit mAb at dilution 1/20

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting