• Product NameMCL1 Rabbit mAb
• Host SpeciesRecombinant Rabbit
• ClonalityMonoclonal antibody
• Clone No.S04-3A4
• IsotypeRabbit IgG
• PurificationAffinity Purified
• ApplicationsWB IF
• Species ReactivityHuman,Mouse,Rat
• Immunogen DescriptionA synthetic peptide of human MCL1
• ConjugatesUnconjugated
• ModificationUnmodification
• Alternative Names    TM; EAT; MCL1L; MCL1S; Mcl-1; BCL2L3; MCL1-ES; bcl2-L-3; mcl1/EAT
• Accession No. Swiss-Prot:Q07820
  GeneID:4170
• UniprotQ07820
• Gene ID4170
• Calculated MW Calculated MW: 37 kDa; Observed MW: 40 kDa
• Concentration 0.3 mg/ml
• Formulation 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA
• Storage Store at 4˚C short term. Aliquot and store at -20˚C long term. Avoid freeze/thaw cycles.

• WB: 1/1000; ICC/IF: 1/50;

Swiss-Prot Acc.Q07820.Involved in the regulation of apoptosis versus cell survival, and in the maintenance of viability but not of proliferation. Mediates its effects by interactions with a number of other regulators of apoptosis. Isoform 1 inhibits apoptosis. Isoform 2 promotes apoptosis.

• Western blot detection of MCL1 in C6 cell lysates using MCL1 Rabbit mAb(1:1000 diluted).Predicted band size:37kDa.Observed band size:40kDa.
• Immunofluorescence of MCL1 (green) in MCF-7 using MCL1 antibody at dilution 1/20, and DAPI(blue)

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting