• Product Namealpha Actinin 4 Rabbit mAb
• Host SpeciesRecombinant Rabbit
• ClonalityMonoclonal antibody
• Clone No.S06-3D7
• IsotypeRabbit IgG
• PurificationAffinity Purified
• ApplicationsWB IF
• Species ReactivityHuman,Mouse,Rat
• Immunogen DescriptionA synthetic peptide of human alpha Actinin 4
• ConjugatesUnconjugated
• ModificationUnmodification
• Alternative Names    FSGS; FSGS1; ACTININ-4
• Accession No. Swiss-Prot:O43707
  GeneID:81
• UniprotO43707
• Gene ID81
• Calculated MW Calculated MW: 105 kDa; Observed MW: 105 kDa
• Concentration 0.3 mg/ml
• Formulation 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA
• Storage Store at 4˚C short term. Aliquot and store at -20˚C long term. Avoid freeze/thaw cycles.

• WB: 1/1000-1/5000; ICC/IF: 1/20-1/50;

Swiss-Prot Acc.O43707.F-actin cross-linking protein which is thought to anchor actin to a variety of intracellular structures. This is a bundling protein (Probable). Probably involved in vesicular trafficking via its association with the CART complex. The CART complex is necessary for efficient transferrin receptor recycling but not for EGFR degradation (PubMed:15772161). Involved in tight junction assembly in epithelial cells probably through interaction with MICALL2. Links MICALL2 to the actin cytoskeleton and recruits it to the tight junctions . May also function as a transcriptional coactivator, stimulating transcription mediated by the nuclear hormone receptors PPARG and RARA (PubMed:22351778).

• Western blot detection of alpha Actinin 4 in K562,Rat Brain,C6,3T3,Hela cell lysates using alpha Actinin 4 Rabbit mAb(1:1000 diluted).Predicted band size:105kDa.Observed band size:105kDa.
• Immunofluorescence of alpha Actinin 4(green) in Hela cells using alpha Actinin 4 Rabbit mAb at dilution 1/200, and DAPI(blue)

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting