• Product NameBAG3 Rabbit mAb
• Host SpeciesRecombinant Rabbit
• ClonalityMonoclonal antibody
• Clone No.S05-6D5
• IsotypeRabbit IgG
• PurificationAffinity Purified
• ApplicationsWB
• Species ReactivityHuman,Mouse,Rat
• Immunogen DescriptionRecombinant protein of human Bag3
• ConjugatesUnconjugated
• ModificationUnmodification
• Alternative Names    BIS; MFM6; BAG-3; CAIR-1
• Accession No. Swiss-Prot:O95817
  GeneID:9531
• UniprotO95817
• Gene ID9531
• Calculated MW Calculated MW: 62 kDa; Observed MW: 80 kDa
• Concentration 0.3 mg/ml
• Formulation 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA
• Storage Store at 4˚C short term. Aliquot and store at -20˚C long term. Avoid freeze/thaw cycles.

• WB: 1/1000;

Swiss-Prot Acc.O95817.Co-chaperone for HSP70 and HSC70 chaperone proteins. Acts as a nucleotide-exchange factor (NEF) promoting the release of ADP from the HSP70 and HSC70 proteins thereby triggering client/substrate protein release. Nucleotide release is mediated via its binding to the nucleotide-binding domain (NBD) of HSPA8/HSC70 where as the substrate release is mediated via its binding to the substrate-binding domain (SBD) of HSPA8/HSC70 (PubMed:9873016, PubMed:27474739). Has anti-apoptotic activity (PubMed:10597216). Plays a role in the HSF1 nucleocytoplasmic transport (PubMed:26159920).

• Western blot detection of Bag3 in K562,Rat Brain cell lysates using Bag3 Rabbit mAb(1:1000 diluted).Predicted band size:62kDa.Observed band size:80kDa.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting