• Product NameCYFIP1 Rabbit mAb
• Host SpeciesRecombinant Rabbit
• ClonalityMonoclonal antibody
• Clone No.S08-9B4
• IsotypeRabbit IgG
• PurificationAffinity Purified
• ApplicationsWB
• Species ReactivityHuman,Mouse,Rat
• Immunogen DescriptionA synthetic peptide of human CYFIP1
• ConjugatesUnconjugated
• ModificationUnmodification
• Alternative Names    SHYC; SRA1; SRA-1; P140SRA-1
• Accession No. Swiss-Prot:Q7L576
  GeneID:23191
• UniprotQ7L576
• Gene ID23191
• Calculated MW Calculated MW: 145 kDa; Observed MW: 145 kDa
• Concentration 0.3 mg/ml
• Formulation 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA
• Storage Store at 4˚C short term. Aliquot and store at -20˚C long term. Avoid freeze/thaw cycles.

• WB: 1/1000-1/5000;

Swiss-Prot Acc.Q7L576.Component of the CYFIP1-EIF4E-FMR1 complex which binds to the mRNA cap and mediates translational repression. In the CYFIP1-EIF4E-FMR1 complex this subunit is an adapter between EIF4E and FMR1. Promotes the translation repression activity of FMR1 in brain probably by mediating its association with EIF4E and mRNA . Regulates formation of membrane ruffles and lamellipodia. Plays a role in axon outgrowth. Binds to F-actin but not to RNA. Part of the WAVE complex that regulates actin filament reorganization via its interaction with the Arp2/3 complex. Actin remodeling activity is regulated by RAC1. Regulator of epithelial morphogenesis. As component of the WAVE1 complex, required for BDNF-NTRK2 endocytic trafficking and signaling from early endosomes . May act as an invasion suppressor in cancers.

• Western blot detection of CYFIP1 in K562,Rat Brain,C6,3T3,Hela cell lysates using CYFIP1 Rabbit mAb(1:1000 diluted).Predicted band size:145kDa.Observed band size:145kDa.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting