• Product NameDKC1 Rabbit mAb
• Host SpeciesRecombinant Rabbit
• ClonalityMonoclonal antibody
• Clone No.S02-2D8
• IsotypeRabbit IgG
• PurificationAffinity Purified
• ApplicationsWB IHC
• Species ReactivityHuman,Mouse,Rat
• Immunogen DescriptionA synthetic peptide of human DKC1
• ConjugatesUnconjugated
• ModificationUnmodification
• Alternative Names    DKC; CBF5; DKCX; NAP57; NOLA4; XAP101
• Accession No. Swiss-Prot:O60832
  GeneID:1736
• UniprotO60832
• Gene ID1736
• Calculated MW Calculated MW: 58 kDa; Observed MW: 58 kDa
• Concentration 0.3 mg/ml
• Formulation 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA
• Storage Store at 4˚C short term. Aliquot and store at -20˚C long term. Avoid freeze/thaw cycles.

• WB: 1/1000-1/5000; IHC: 1/20;

Swiss-Prot Acc.O60832.Isoform 1: Catalytic subunit of H/ACA small nucleolar ribonucleoprotein (H/ACA snoRNP) complex, which catalyzes pseudouridylation of rRNA (PubMed:25219674). This involves the isomerization of uridine such that the ribose is subsequently attached to C5, instead of the normal N1 (PubMed:25219674). Each rRNA can contain up to 100 pseudouridine ('psi') residues, which may serve to stabilize the conformation of rRNAs. Required for ribosome biogenesis and telomere maintenance (PubMed:19179534, PubMed:25219674). Also required for correct processing or intranuclear trafficking of TERC, the RNA component of the telomerase reverse transcriptase (TERT) holoenzyme (PubMed:19179534).

• Western blot detection of DKC1 in K562,C6,3T3,Hela cell lysates using DKC1 Rabbit mAb(1:1000 diluted).Predicted band size:58kDa.Observed band size:58kDa.
• Immunohistochemistry of DKC1 in paraffin-embedded Human breast cancer tissue using DKC1 Rabbit mAb at dilution 1/50

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting