• Product NameDNA Ligase IV Rabbit mAb
• Host SpeciesRecombinant Rabbit
• ClonalityMonoclonal antibody
• Clone No.S08-3B4
• IsotypeRabbit IgG
• PurificationAffinity Purified
• ApplicationsWB
• Species ReactivityHuman
• Immunogen DescriptionRecombinant protein of human DNA Ligase IV
• ConjugatesUnconjugated
• ModificationUnmodification
• Alternative Names    LIG4S
• Accession No. Swiss-Prot:P49917
  GeneID:3981
• UniprotP49917
• Gene ID3981
• Calculated MW Calculated MW: 104 kDa; Observed MW: 104 kDa
• Concentration 0.3 mg/ml
• Formulation 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA
• Storage Store at 4˚C short term. Aliquot and store at -20˚C long term. Avoid freeze/thaw cycles.

• WB: 1/1000

Swiss-Prot Acc.P49917.Efficiently joins single-strand breaks in a double-stranded polydeoxynucleotide in an ATP-dependent reaction. Involved in DNA non-homologous end joining (NHEJ) required for double-strand break repair and V(D)J recombination. The LIG4-XRCC4 complex is responsible for the NHEJ ligation step, and XRCC4 enhances the joining activity of LIG4. Binding of the LIG4-XRCC4 complex to DNA ends is dependent on the assembly of the DNA-dependent protein kinase complex DNA-PK to these DNA ends.

• Western blot detection of DNA Ligase IV in K562,U251MG,HepG2,MCF7 cell lysates using DNA Ligase IV Rabbit mAb(1:500 diluted).Predicted band size:104kDa.Observed band size:104kDa.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting