• Product NameeIF2A Rabbit mAb
• Host SpeciesRecombinant Rabbit
• ClonalityMonoclonal antibody
• Clone No.S08-8E2
• IsotypeRabbit IgG
• PurificationAffinity Purified
• ApplicationsWB IF
• Species ReactivityHuman,Mouse,Rat
• Immunogen DescriptionA synthetic peptide of human eIF2A
• ConjugatesUnconjugated
• ModificationUnmodification
• Alternative Names    CDA02; EIF-2A; MST089; MSTP004; MSTP089
• Accession No. Swiss-Prot:Q9BY44
  GeneID:83939
• UniprotQ9BY44
• Gene ID83939
• Calculated MW Calculated MW: 65 kDa; Observed MW: 65 kDa
• Concentration 0.3 mg/ml
• Formulation 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA
• Storage Store at 4˚C short term. Aliquot and store at -20˚C long term. Avoid freeze/thaw cycles.

• WB: 1/1000; ICC/IF: 1/20

Swiss-Prot Acc.Q9BY44.Functions in the early steps of protein synthesis of a small number of specific mRNAs. Acts by directing the binding of methionyl-tRNAi to 40S ribosomal subunits. In contrast to the eIF-2 complex, it binds methionyl-tRNAi to 40S subunits in a codon-dependent manner, whereas the eIF-2 complex binds methionyl-tRNAi to 40S subunits in a GTP-dependent manner.

• Western blot detection of eIF2A in LnCap,K562,U251MG cell lysates using eIF2A Rabbit mAb(1:500 diluted).Predicted band size:65kDa.Observed band size:65kDa.
• Immunofluorescence of eIF2A (green) in MCF-7 using eIF2A antibody at dilution 1/20, and DAPI(blue)

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting