• Product NameEWSR1 Rabbit mAb
• Host SpeciesRecombinant Rabbit
• ClonalityMonoclonal antibody
• Clone No.S09-3A7
• IsotypeRabbit IgG
• PurificationAffinity Purified
• ApplicationsWB IF
• Species ReactivityHuman,Mouse,Rat
• Immunogen DescriptionA synthetic peptide of human EWSR1
• ConjugatesUnconjugated
• ModificationUnmodification
• Alternative Names    EWS; EWS-FLI1; bK984G1.4
• Accession No. Swiss-Prot:Q01844
  GeneID:2130
• UniprotQ01844
• Gene ID2130
• Calculated MW Calculated MW: 69 kDa; Observed MW: 85 kDa
• Concentration 0.3 mg/ml
• Formulation 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA
• Storage Store at 4˚C short term. Aliquot and store at -20˚C long term. Avoid freeze/thaw cycles.

• WB: 1/2000-1/10000; ICC/IF: 1/50-1/200

Swiss-Prot Acc.Q01844.Might normally function as a transcriptional repressor. EWS-fusion-proteins (EFPS) may play a role in the tumorigenic process. They may disturb gene expression by mimicking, or interfering with the normal function of CTD-POLII within the transcription initiation complex. They may also contribute to an aberrant activation of the fusion protein target genes.SitesFeature keyPosition(s)Description

• Western blot detection of EWSR1 in MCF7,3T3,Hela cell lysates using EWSR1 Rabbit mAb(1:1000 diluted).Predicted band size:69KDa.Observed band size:85KDa.
• Immunofluorescence of EWSR1(green) in Hela cells using EWSR1 Rabbit mAb at dilution 1/50, and DAPI(blue)

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting