• Product NameGNA13 Rabbit mAb
• Host SpeciesRecombinant Rabbit
• ClonalityMonoclonal antibody
• Clone No.S08-4F2
• IsotypeRabbit IgG
• PurificationAffinity Purified
• ApplicationsWB IHC
• Species ReactivityHuman,Mouse,Rat
• Immunogen DescriptionA synthetic peptide of human G protein alpha 13
• ConjugatesUnconjugated
• ModificationUnmodification
• Alternative Names    guanine nucleotide binding protein (G protein), alpha 13;G13
• Accession No. Swiss-Prot:Q14344
  GeneID:10672
• UniprotQ14344
• Gene ID10672
• Calculated MW Calculated MW: 44 kDa; Observed MW: 44 kDa
• Concentration 0.3 mg/ml
• Formulation 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA
• Storage Store at 4˚C short term. Aliquot and store at -20˚C long term. Avoid freeze/thaw cycles.

• WB: 1/1000-1/5000; IHC: 1/200

Swiss-Prot Acc.Q14344.Guanine nucleotide-binding proteins (G proteins) are involved as modulators or transducers in various transmembrane signaling systems (PubMed:15240885, PubMed:16787920, PubMed:16705036, PubMed:27084452). Activates effector molecule RhoA by binding and activating RhoGEFs (ARHGEF1/p115RhoGEF, ARHGEF11/PDZ-RhoGEF and ARHGEF12/LARG) (PubMed:15240885, PubMed:12515866). GNA13-dependent Rho signaling subsequently regulates transcription factor AP-1 (activating protein-1) . Promotes tumor cell invasion and metastasis by activating RhoA/ROCK signaling pathway (PubMed:16787920, PubMed:16705036, PubMed:27084452). Inhibits CDH1-mediated cell adhesion in process independent from Rho activation (PubMed:11976333).

• Western blot detection of GNA13 [KO Validated] in Hela,Jurkat cell lysates using GNA13 Rabbit mAb [KO Validated](1:500 diluted).Predicted band size:44kDa.Observed band size:44kDa.
• Immunohistochemistry of G protein alpha 13 in paraffin-embedded Human Cholangiocarcinoma using G protein alpha 13 Rabbit mAb at dilution 1/50

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting