• Product NameG3BP Rabbit mAb
• Host SpeciesRecombinant Rabbit
• ClonalityMonoclonal antibody
• Clone No.S09-5B9
• IsotypeRabbit IgG
• PurificationAffinity Purified
• ApplicationsWB IF
• Species ReactivityHuman
• Immunogen DescriptionA synthetic peptide of human G3BP
• ConjugatesUnconjugated
• ModificationUnmodification
• Alternative Names    G3BP; HDH-VIII
• Accession No. Swiss-Prot:Q13283
  GeneID:10146
• UniprotQ13283
• Gene ID10146
• Calculated MW Calculated MW: 52 kDa; Observed MW: 68 kDa
• Concentration 0.3 mg/ml
• Formulation 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA
• Storage Store at 4˚C short term. Aliquot and store at -20˚C long term. Avoid freeze/thaw cycles.

• WB: 1/2000-1/10000; ICC/IF: 1/50-1/200;

Swiss-Prot Acc.Q13283.ATP- and magnesium-dependent helicase (PubMed:9889278). Unwinds preferentially partial DNA and RNA duplexes having a 17 bp annealed portion and either a hanging 3' tail or hanging tails at both 5'- and 3'-ends (PubMed:9889278). Unwinds DNA/DNA, RNA/DNA, and RNA/RNA substrates with comparable efficiency (PubMed:9889278). Acts unidirectionally by moving in the 5' to 3' direction along the bound single-stranded DNA (PubMed:9889278). Phosphorylation-dependent sequence-specific endoribonuclease in vitro (PubMed:11604510). Cleaves exclusively between cytosine and adenine and cleaves MYC mRNA preferentially at the 3'-UTR (PubMed:11604510). May be a regulated effector of stress granule assembly (PubMed:12642610, PubMed:20180778).

• Western blot detection of G3BP in K562,C6,Hela cell lysates using G3BP Rabbit mAb(1:1000 diluted).Predicted band size:52kDa.Observed band size:68kDa.
• Immunofluorescence of G3BP(green) in Hela cells using G3BP Rabbit mAb at dilution 1/200, and DAPI(blue)

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting