• Product NameGlutaminase Rabbit mAb
• Host SpeciesRecombinant Rabbit
• ClonalityMonoclonal antibody
• Clone No.S08-3D8
• IsotypeRabbit IgG
• PurificationAffinity Purified
• ApplicationsWB
• Species ReactivityHuman,Mouse,Rat
• Immunogen DescriptionRecombinant protein of human Glutaminase C
• ConjugatesUnconjugated
• ModificationUnmodification
• Alternative Names    GAC; GAM; KGA; GLS1; AAD20
• Accession No. Swiss-Prot:O94925
  GeneID:2744
• UniprotO94925
• Gene ID2744
• Calculated MW Calculated MW: 65 kDa; Observed MW: 68 kDa
• Concentration 0.3 mg/ml
• Formulation 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA
• Storage Store at 4˚C short term. Aliquot and store at -20˚C long term. Avoid freeze/thaw cycles.

• WB: 1/1000;

Swiss-Prot Acc.O94925.Catalyzes the first reaction in the primary pathway for the renal catabolism of glutamine. Plays a role in maintaining acid-base homeostasis. Regulates the levels of the neurotransmitter glutamate in the brain. Isoform 2 lacks catalytic activity.CautionIsoform 3 is predicted to be expressed at very low levels due to a premature stop codon in the mRNA, leading to nonsense-mediated mRNA decay (PubMed:14759258), but has been shown to be well expressed (PubMed:17940881 and PubMed:11015561) and the encoded protein is detected in mitochondria (PubMed:22228304).

• Western blot detection of Glutaminase in K562,3T3,Hela cell lysates using Glutaminase Rabbit mAb(1:1000 diluted).Predicted band size:74kDa.Observed band size:68kDa.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting