• Product NameHistone H3 Rabbit mAb
• Host SpeciesRecombinant Rabbit
• ClonalityMonoclonal antibody
• Clone No.S06-4I8
• IsotypeRabbit IgG
• PurificationAffinity Purified
• ApplicationsWB IHC
• Species ReactivityHuman,Mouse,Rat
• Immunogen DescriptionA synthetic peptide of human Histone H3
• ConjugatesUnconjugated
• ModificationUnmodification
• Alternative Names    H3/A; H3FA
• Accession No. Swiss-Prot:P68431
  GeneID:8350
• UniprotP68431
• Gene ID8350
• Calculated MW Calculated MW: 15 kDa; Observed MW: 17 kDa
• Concentration 0.3 mg/ml
• Formulation 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA
• Storage Store at 4˚C short term. Aliquot and store at -20˚C long term. Avoid freeze/thaw cycles.

• WB: 1/1000; IHC: 1/50;

Swiss-Prot Acc.P68431.Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.MiscellaneousThis histone is only present in mammals and is enriched in acetylation of Lys-15 and dimethylation of Lys-10 (H3K9me2).CautionThe original paper reporting lysine deamination at Lys-5 by LOXL2 has been retracted due to inappropriate manipulation of figure data (PubMed:22483618, PubMed:27392148). However, this modification was confirmed in a subsequent publication (PubMed:27735137).

• Western blot detection of Histone H3 in Jurkat,THP-1,C6,3T3 cell lysates using Histone H3 Rabbit mAb(1:1000 diluted).Predicted band size:15KDa.Observed band size:17KDa.
• Immunohistochemistry of Histone H3 in paraffin-embedded Human lung cancer tissue using Histone H3 Rabbit mAb at dilution 1/50

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting