• Product NameJunctional Adhesion Molecule 1 Rabbit mAb
• Host SpeciesRecombinant Rabbit
• ClonalityMonoclonal antibody
• Clone No.S04-7E5
• IsotypeRabbit IgG
• PurificationAffinity Purified
• ApplicationsWB IHC
• Species ReactivityHuman
• Immunogen DescriptionA synthetic peptide of human Junctional Adhesion Molecule 1
• ConjugatesUnconjugated
• ModificationUnmodification
• Alternative Names    JAM; KAT; JAM1; JAMA; JCAM; CD321; PAM-1
• Accession No. Swiss-Prot:Q9Y624
  GeneID:50848
• UniprotQ9Y624
• Gene ID50848
• Calculated MW Calculated MW: 33 kDa; Observed MW: 33 kDa
• Concentration 0.3 mg/ml
• Formulation 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA
• Storage Store at 4˚C short term. Aliquot and store at -20˚C long term. Avoid freeze/thaw cycles.

• WB: 1/2000; IHC: 1/50

Swiss-Prot Acc.Q9Y624.Seems to play a role in epithelial tight junction formation. Appears early in primordial forms of cell junctions and recruits PARD3 (PubMed:11489913). The association of the PARD6-PARD3 complex may prevent the interaction of PARD3 with JAM1, thereby preventing tight junction assembly . Plays a role in regulating monocyte transmigration involved in integrity of epithelial barrier . Ligand for integrin alpha-L/beta-2 involved in memory T-cell and neutrophil transmigration (PubMed:11812992). Involved in platelet activation (PubMed:10753840).

• Western blot detection of Junctional Adhesion Molecule 1/JAM-A in K562,PC3 cell lysates using Junctional Adhesion Molecule 1/JAM-A Rabbit mAb(1:1000 diluted).Predicted band size:33kDa.Observed band size:33kDa.
• Immunohistochemistry of Junctional Adhesion Molecule 1 in paraffin-embedded Human lung cancer tissue using Junctional Adhesion Molecule 1 Rabbit mAb at dilution 1/50

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting