• Product NameMAGEA3 Rabbit mAb
• Host SpeciesRecombinant Rabbit
• ClonalityMonoclonal antibody
• Clone No.S07-8A2
• IsotypeRabbit IgG
• PurificationAffinity Purified
• ApplicationsWB
• Species ReactivityHuman
• Immunogen DescriptionRecombinant protein of human MAGEA3
• ConjugatesUnconjugated
• ModificationUnmodification
• Alternative Names    HIP8; HYPD; CT1.3; MAGE3; MAGEA6
• Accession No. Swiss-Prot:P43357
  GeneID:4102
• UniprotP43357
• Gene ID4102
• Calculated MW Calculated MW: 35 kDa; Observed MW: 45 kDa
• Concentration 0.3 mg/ml
• Formulation 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA
• Storage Store at 4˚C short term. Aliquot and store at -20˚C long term. Avoid freeze/thaw cycles.

• WB: 1/1000

Swiss-Prot Acc.P43357.Proposed to enhance ubiquitin ligase activity of RING-type zinc finger-containing E3 ubiquitin-protein ligases. May enhance ubiquitin ligase activity of TRIM28 and stimulate p53/TP53 ubiquitination by TRIM28. Proposed to act through recruitment and/or stabilization of the Ubl-conjugating enzyme (E2) at the E3:substrate complex. May play a role in embryonal development and tumor transformation or aspects of tumor progression. In vitro promotes cell viability in melanoma cell lines. Antigen recognized on a melanoma by autologous cytolytic T-lymphocytes.

• Western blot detection of MAGEA3 in Hela cell lysates using MAGEA3 Rabbit mAb(1:1000 diluted).Predicted band size:35kDa.Observed band size:45kDa.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting