• Product NameMLKL Rabbit mAb
• Host SpeciesRecombinant Rabbit
• ClonalityMonoclonal antibody
• Clone No.S07-5G9
• IsotypeRabbit IgG
• PurificationAffinity Purified
• ApplicationsWB
• Species ReactivityHuman
• Immunogen DescriptionRecombinant protein of human MLKL
• ConjugatesUnconjugated
• ModificationUnmodification
• Alternative Names    hMLKL
• Accession No. Swiss-Prot:Q8NB16
  GeneID:197259
• UniprotQ8NB16
• Gene ID197259
• Calculated MW Calculated MW: 55 kDa; Observed MW: 55 kDa
• Concentration 0.3 mg/ml
• Formulation 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA
• Storage Store at 4˚C short term. Aliquot and store at -20˚C long term. Avoid freeze/thaw cycles.

• WB: 1/1000

Swiss-Prot Acc.Q8NB16.Pseudokinase that plays a key role in TNF-induced necroptosis, a programmed cell death process. Activated following phosphorylation by RIPK3, leading to homotrimerization, localization to the plasma membrane and execution of programmed necrosis characterized by calcium influx and plasma membrane damage. Does not have protein kinase activity (PubMed:22265413, PubMed:22265414, PubMed:22421439, PubMed:24316671). Binds to highly phosphorylated inositol phosphates such as inositolhexakisphosphate (InsP6) which is essential for its necroptotic function (PubMed:29883610).

• Western blot detection of MLKL in HT-29 cell lysates using MLKL Rabbit mAb(1:1000 diluted).Predicted band size:55KDa.Observed band size:55KDa.
• Western blot detection of MLKL in U251MG,Hela cell lysates using MLKL Rabbit mAb(1:500 diluted).Predicted band size:55kDa.Observed band size:55kDa.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting